Mutagenesis Using Robertson’s Mutator Lines and Consequent Insertions at the Adh1 Gene in Maize
The Mu transposons in maize and their unique behaviors have been reviewed recently (14), as have results on the structure, function, and regulation of the alcohol dehydrogenase-1 gene (Adh1) in maize (11). My purpose is to review concisely our published and unpublished work on Mu1 and Mu3 insertional mutants at Adh1 and to discuss what I now take to be interesting or useful observations concerning mutagenesis using Mu.
KeywordsTransposable Element Inverted Repeat Mutator Line Allyl Alcohol Mutator Element
Unable to display preview. Download preview PDF.
- 1.Alleman, M. (1985) The Mu transposons of maize. Ph.D. dissertation, University of California, Berkeley.Google Scholar
- 4.Bennetzen, J.L. (1987) Covalent DNA modification and the regulation of mutator element. Molec. Gen. Genet. 208:454–451.Google Scholar
- 5.Bennetzen, J.L., R.P. Fracasso, D.W. Morris, D. Robertson, and M.J. Skogen-Hagenson (1986) Concomitant regulation of Mu1 transposition and mutator activity in maize. Molec. Gen. Genet. (in press).Google Scholar
- 8.Chen, C.-H., M. Freeling, and A. Merckelbach (1986) Enzymatic and morphological consequences of Ds excisions from maize Adh1. Maydica 31:93-108 (the McClintock commemorative issue).Google Scholar
- 10.Dobzahnsky, T. (1966) Determination and indeterminism in biological evolution. In Philosophical Problems in Biology, V.E. Smith, ed. St. John’s University Press.Google Scholar
- 12.Goldschmidt, R.B. (1952) Evolution as viewed by one geneticist. Amer. Sci. 40:84–135.Google Scholar
- 13.Lillis, M., A. Spielmann, and R.B. Simpson (1985) Transfer of a Robertson’s mutator element to tomato and tobacco cells. In Plant Genetics, M. Freeling, ed. Alan R. Liss, New York, pp. 213–224.Google Scholar
- 19.Robertson, D.S., P.S. Stinard, J.G. Wheeler, and D.W. Morris (1985) Genetic and molecular studies on germinal and somatic mutability in Mutator-induced aleurone mutants of maize. In Plant Genetics, M. Freeling, ed. Alan R. Liss, New York, pp. 317–331.Google Scholar
- 23.Taylor, L.P., V. Chandler, and V. Walbot (1986) Insertion of 1.4KB and 1.7KB elements into the Bronzel gene of Zea mays L. Maydica 31:31–45.Google Scholar