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Transposition of Ac in Tobacco

  • Barbara Baker
  • George Coupland
  • Reinhard Hehl
  • Nina Fedoroff
  • Horst Lörz
  • Peter Czernilofsky
  • Peter Starlinger
  • Jeff Schell
Part of the Basic Life Sciences book series (BLSC, volume 47)

Abstract

The maize transposable element Activator (Ac) was first identified and studied genetically by Barbara McClintock (15; for review, see Ref. 9). The Ac element is capable of transposing autonomously, and it can also trans-activate the transposition of a group of elements collectively designated Dissociation (Ds) elements. Ac and Ds elements comprise a maize transposon family. Many elements of this family have been cloned and subjected to structural analysis (3,6,10). The Ac element is a small, 4.6-kilobase (kb) transposon that has an 11-base-pair (bp) terminal inverted repetition and generates an 8-bp duplication upon insertion. Sequence analysis of the element has revealed the presence of three major open reading frames (ORFs) (16,19). Recently, an RNA transcript of 3.5 kb was identified and found exclusively in maize lines that carried an active Ac (13). Overlapping cDNA clones spanning most of the mRNA were sequenced. The transcript contains a 600–700 nucleotide long untranslated leader, an open reading frame encoding 807 amino acids, and an untranslated 3′ sequence of 239 nucleotides. Four introns with a combined length of 654 bases are removed from the primary transcript.

Keywords

Callus Line Restriction Enzyme Fragment Major Open Reading Frame BamHI Restriction Enzyme Site Progenitor Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Barbara Baker
    • 1
  • George Coupland
    • 2
  • Reinhard Hehl
    • 1
  • Nina Fedoroff
    • 3
  • Horst Lörz
    • 4
  • Peter Czernilofsky
    • 5
  • Peter Starlinger
    • 2
  • Jeff Schell
    • 4
  1. 1.Plant Gene Expression CenterU. S. Department of AgricultureAlbanyUSA
  2. 2.Institut für GenetikUniversitat zu KölnKöln 41Federal Republic of Germany
  3. 3.Department of EmbryologyCarnegie Institution of WashingtonBaltimoreUSA
  4. 4.Max-Planck-Institut für ZüchtungsforschungKöln 30Federal Republic of Germany
  5. 5.California Biotechnology, Inc.Mountain ViewUSA

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