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Establishing the Order of Human Chromosome-Specific DNA Fragments

  • A. V. Carrano
Part of the Basic Life Sciences book series (BLSC, volume 46)

Abstract

The goal of the human genome project is to decipher the human genetic information (i.e. determine the sequence of the DNA bases) for the benefit of mankind. Questions about this project center on emphasis and approach, not on the scientific value. One important question focuses on the need to sequence the entire genome rather than subsets of the genome, since it is thought that more than 80 percent of the human genome may not contain useful genetic information.

Keywords

Human Chromosome Fragment Size Hybrid Cell Large Fragment Cosmid Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Brenner, S., Cesareni, G., and Karn, J., 1982, Phasmids: hybrids between ColE1 plasmids and E. coli bacteriophage lambda, Gene, 17: 27.PubMedCrossRefGoogle Scholar
  2. Carle, G. F., Frank, M., and Olson, M. V., 1986, Electrophoretic separations of large DNA molecules by periodic inversion of the electric field, Science, 232: 65.PubMedCrossRefGoogle Scholar
  3. Carrano, A. V., Gray, J. W., Langlois, R. G., Burkhart-Schultz, K. J., and Van Dilla, M. A., 1979, Measurement and purification of human chromosomes by flow cytometry and sorting, Proc. Natl. Acad. Sci. U.S.A., 76: 1382.PubMedCrossRefGoogle Scholar
  4. Chu, G., Vollrath, D., and Davis, R. W., 1986, Separation of large DNA molecules by contour-clamped homogeneous electric fields, Science, 234: 1582.PubMedCrossRefGoogle Scholar
  5. Connell, C., Fung, S., Heiner, C., Bridgham, J., Chakerian, V., Heron, E., Jones, B., Menchen, S., Mordan, W., Raff, M., Recknor, M., Smith, L., Springer, J., Woo, S., and Hunkapiller, M., 1987, Automated DNA sequence analysis, Bio Techniques, 5: 342.Google Scholar
  6. Coulson, A., Sulston, J., Brenner, S., and Karn, J., 1986, Toward a physical map of the genome of the nematode Caenorhabditis elegans, Proc. Natl. Acad. Sci. U.S.A., 83: 7821.PubMedCrossRefGoogle Scholar
  7. Gray, J. W., Carrano, A. V., Steinmetz, L. L., Van Dilla, M. A., Moore, D. H., Mayall, B.H., and Mendelsohn, M.L., 1975, Chromosome measurement and sorting by flow systems, Proc. Natl. Acad. Sci. U.S.A., 72: 1231.PubMedCrossRefGoogle Scholar
  8. Gray, J. W., Dean, P. N., Fuscoe, J. C., Peters, D. C., Trask, B. J., van den Engh, G. J., and Van Dilla, M. A., 1987, High-speed chromosome sorting, Science, 238: 323.PubMedCrossRefGoogle Scholar
  9. Kohara, Y., Akiyama, K., and Isono, K., 1987, The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library, Cell, 50: 495.PubMedCrossRefGoogle Scholar
  10. Langlois, R. G., Yu, L.-C., Gray, J. W., and Carrano, A. V., 1982, Quantitative karyotyping of human chromosomes by dual beam flow cytometry. Proc. Natl. Acad. Sci. U.S.A., 79: 7876.PubMedCrossRefGoogle Scholar
  11. Little, P. F. R., 1987, Choice and use of cosmid vectors, in: “DNA Cloning,” Vol. III, D. M. Glover, ed., IRL Press, Ltd., Oxford.Google Scholar
  12. Mayall, B. H., Carrano, A. V., Moore, II, D. H., Ashworth, L. K., Bennett, D. E., and Mendelsohn, M. L., 1984, The DNA-based human karyotype, Cytometry, 5: 376.PubMedCrossRefGoogle Scholar
  13. McKenney, K., Shimatake, H., Court, D., Schmeissner, U., Brady, C., and Rosenberg, M., 1980, A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase, in: “GeneAmplification and Analysis,” Vol. 2, Chirikjan, J. G. and Papas, T., eds., Elsevier/North-Holland, Publ.Google Scholar
  14. Olson, M. V., Dutchik, J. E., Graham, M. Y., Brodeur, G. M., Helms, C., Frank, M., MacCollin, M., Scheinman, R., and Frank, T., 1986, Random-clone strategy for genomic restriction mapping in yeast, Proc. Natl. Acad. Sci. U.S.A., 83: 7826.PubMedCrossRefGoogle Scholar
  15. Poustka, A., Pohl, T. M., Barlow, D. P., Frischauf, A. -M., and Lehrach, H., 1987, Construction and use of human chromosome jumping libraries from NotI-digested DNA, Nature, 325: 353.PubMedCrossRefGoogle Scholar
  16. Schwartz, D. C., and Cantor, C. R., 1984, Separation of yeast chromosome-sized DNAs by pulsed field gradient electrophoresis, Cell, 37:67.PubMedCrossRefGoogle Scholar
  17. Siciliano, M. J., Carrano, A. V., and Thompson, L. H., 1986a, Assignment of a human DNA-repair gene associated with sister-chromatid exchange to chromosome 19, Mutat. Res., 174: 303.CrossRefGoogle Scholar
  18. Siciliano, M.J., Carrano, A.V. and Thompson, L.H., 1986b, DNA repair gene on chromosome 16 and third repair gene on chromosome 19, Am. J. Hum. Genet., 39: A42.Google Scholar
  19. Smith, C. L., Econome, J. G., Schutt, A., Klco, S., and Canton, C. R., 1987, A physical map of the Escherichia coli K12 genome, Science, 236: 1448.PubMedCrossRefGoogle Scholar
  20. Smith, L. M., Fung, S., Hunkapiller, M. W., Hunkapiller, T. J., and Hood, L. E., 1985, The synthesis of oligonucleotides containing an aliphatic amino group at the 5’ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis, Nucl. Acids Res., 13: 2399.PubMedCrossRefGoogle Scholar
  21. Stueber, D., and Bujard, H., 1982, Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes, EMBO J., 1: 1399.PubMedGoogle Scholar
  22. Thompson, L. H., Brookman, K. W., Minkler, J. L., Fuscoe, J. C., Henning, K. A., and Carrano, A. V., 1985, DNA-mediated transfer of human DNA repair gene that controls sister-chromatid exchange, Molec. Cell Biol., 5: 881.PubMedGoogle Scholar
  23. Van Dilla, M. A., Deaven, L. L., Albright, K. L., Allen, N. A., Aubuchon, M. R., Bartholdi, M. F., Brown, N. C., Campbell, E. W., Carrano, A. V., Clark, L. M., Cram, L. S., Fuscoe, J. C., Gray, J. W., Hildebrand, C. E., Jackson, P. J., Jett, J. H., Longmire, J. L., Lozes, C. R., Luedemann, M. L., Martin, J. C., McNinch, J. S., Meincke, L. J., Mendelsohn, M. L., Meyne, J., Moyzis, R. K., Munk, A. C., Perlman, J., Peters, D. C., Silva, A. J., and Trask, B. J., 1986, Human chromosome-specific DNA libraries: construction and availability, Biotechnology, 4: 537.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • A. V. Carrano
    • 1
  1. 1.Biomedical Sciences Division L-452Lawrence Livermore National LaboratoryLivermoreUSA

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