DNA Sequencing Technology
DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year. With the development of automated fluorescence and radiometric sequencing methods, it will be possible to generate considerably more data per person. In this paper we also will describe the fundamental principles of automated sequencing by fluorescence.
KeywordsSanger Sequencing Beta Emission Single Lane Acrylamide Solution Difficult Region
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- 1.A. Maxam and W. Gilbert, Sequencing end-labeled DNA with base specific chemical cleavages, Meth. in Enzymol. 65:500 (1980).Google Scholar
- 4.E. Strauss, J. A. Kobori, G. Siu, and L. Hood, Specific-primer-directed DNA sequencing, Anal. Biochem. 154: 353 (1986).Google Scholar
- 6.M. Hattori and Y. Sakaki, Dideoxy sequencing method using denatured plasmid templates, Anal. Biochem. 152: 232 (1986).Google Scholar
- 7.R. Zagursky, K. Baumeister, N. Lomax, and M. Berman, Rapid and easy sequencing of large linear double stranded DNA and supercoiled plasmid DNA, Gene Anal. Tech. 2: 89 (1985).Google Scholar
- 9.D. Ornstein and M. A. Kashdan, Sequencing DNA using 35S-labeling: a troubleshooting guide, BioTechniques 3: 476 (1985).Google Scholar