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DNA Sequencing Technology

  • Maurice A. Kashdan
  • George L. Trainor
Conference paper
Part of the Basic Life Sciences book series (BLSC, volume 46)

Abstract

DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year. With the development of automated fluorescence and radiometric sequencing methods, it will be possible to generate considerably more data per person. In this paper we also will describe the fundamental principles of automated sequencing by fluorescence.

Keywords

Sanger Sequencing Beta Emission Single Lane Acrylamide Solution Difficult Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Maurice A. Kashdan
    • 1
  • George L. Trainor
    • 2
  1. 1.E. I. duPont de Nemours & Co., Inc.BostonUSA
  2. 2.E. I. duPont de Nemours & Co., Inc.WilmingtonUSA

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