Human Progesterone Receptors A and B: Two Independent, Functional Forms
The use of progesterone receptor (PR) measurements and progestins in the treatment of breast cancers (reviewed in reference 1) and the use of progesterone antagonists in contraception (2) has required an understanding of the actions of these hormones at a basic molecular level. To study the structure and function of human progesterone receptors (hPR), we have used T47Dco, a PR-rich human breast cancer cell line (3), and in situ photoaffinity labeling (4,5); we have also purified the PR from T47D cells and have made anti-PR monoclonal antibodies (6). This chapter describes some of our recent studies using these methods (7) to analyze the structure of hPR.
KeywordsProgesterone Receptor T47D Cell Photoaffinity Label Steroid Binding Protein Progesterone Antagonist
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