Immunocytochemistry of Estrogen and Progestin Receptors in the Primate Reproductive Tract

  • Robert M. Brenner
  • Maryanne C. McClellan
  • Neal B. West
Part of the Serono Symposia, USA book series (SERONOSYMP)


The central focus of our research program, since its inception, has been on steroid receptors as components of regulatory mechanisms that control tissue structure and function in the reproductive tract in female primates. Our approach has been to correlate fluctuations in the levels of receptors with the morphological and physiological effects of the gonadal steroids. Our long-term goal is to deepen our understanding of how steroids bring about the various transformations that occur in the primate reproductive tract during the individual’s life history. Our current research depends heavily on immunocytochemical techniques that have come to maturity in our laboratory over the last few years (1–3). Our findings to date support the hypothesis (4) that there are significant stromal-epithelial interactions involved in steroid hormone action in the adult reproductive tract. Our findings also support the view that in vivo, the bulk of the estrogen receptor (ER) and progestin receptor (PR) are located in target cell nuclei, even in the absence of ligand (5–7).


Reproductive Tract Glandular Epithelium Cynomolgus Macaque Glandular Epithelial Cell Progestin Receptor 


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  1. 1.
    McClellan MC, West NB, Tacha DE, Greene GL, Brenner RM. Immunocytochemical localization of estrogen receptors in the macaque reproductive tract with monoclonal antiestrophilins. Endocrinology 1984; 114: 2002–14.PubMedCrossRefGoogle Scholar
  2. 2.
    Brenner RM, West NB. Immunocytochemistry of the progestin receptor in the reproductive tract of female macaques [Abstract]. Program and Abstracts of the 69th Annual Meeting of The Endocrine Society, Indianapolis, IN, 1987: 79.Google Scholar
  3. 3.
    Bentley JP, Brenner RM, Linstedt AD, et al. Increased hyaluronate and collagen biosynthesis and fibroblast estrogen receptors in macaque sex skin. J Invest Dermatol 1986; 87: 668–73.PubMedCrossRefGoogle Scholar
  4. 4.
    Cunha GR, Chung LWK, Shannon JM, Taguchi O, Fujii H. Hormone-induced morphogenesis and growth: role of mesenchymal-epithelial interactions. Recent Prog Horm Res 1983; 39: 559–98.PubMedGoogle Scholar
  5. 5.
    King WJ, Greene GL. Monoclonal antibodies localize oestrogen receptor in the nuclei of target cells. Nature 1984; 307: 745–7.PubMedCrossRefGoogle Scholar
  6. 6.
    Welshons WV, Lieberman ME, Gorski J. Nuclear localization of unoccupied oestrogen receptors. Nature 1984; 307: 747–9.PubMedCrossRefGoogle Scholar
  7. 7.
    Gravanis A, Gurpide E. Enucleation of human endometrial cells: nucleo-cytoplasmic distribution of DNA polymerase alpha and estrogen receptor. J Steroid Biochem 1986; 24: 469–74.PubMedCrossRefGoogle Scholar
  8. 8.
    Resnik R. The endocrine regulation of uterine blood flow in the nonpregnant uterus: a review. Am J Obstet Gynecol 1981; 140: 151.PubMedGoogle Scholar
  9. 9.
    Brenner RM, West NB. Hormonal regulation of the reproductive tract in female mammals. Annu Rev Physiol 1975; 37: 273–302.PubMedCrossRefGoogle Scholar
  10. 10.
    Brenner RM, West NB, Norman RL, Sandow BA, Verhage HG. Progesterone suppression of the estradiol receptor in the reproductive tract of macaques, cats and hamsters. Adv Exp Med Biol 1979; 117: 173–96.PubMedGoogle Scholar
  11. 11.
    Katzenellenbogen BS. Dynamics of steroid hormone receptor action. Annu Rev Physiol 1980; 42: 17–36.PubMedCrossRefGoogle Scholar
  12. 12.
    Leavitt WW, MacDonald RG, Okulicz WC. Hormonal regulation of estrogen and progesterone receptor systems. In: Litwack G, ed. Biochemical actions of hormones; vol X. New York: Academic Press, 1983: 323–56.Google Scholar
  13. 13.
    West NB, McClellan MC, Sternfeld MD, Brenner RM. Immunocytochemistry versus binding assays of the estrogen receptor in the reproductive tract of spayed and hormone treated macaques. Endocrinology 1987; 121: 1789–800.PubMedCrossRefGoogle Scholar
  14. 14.
    McClellan M, West NB, Brenner RM. Immunocytochemical localization of estrogen receptors in the macaque endometrium during the lutealfollicular transition. Endocrinology 1986; 119: 2467–75.PubMedCrossRefGoogle Scholar
  15. 15.
    Sandow BA, West NB, Norman RL, Brenner RM. Hormonal control of apoptosis in hamster uterine luminal epithelium. Am J Anat 1979; 156: 15–36.PubMedCrossRefGoogle Scholar
  16. 16.
    West NB, Brenner RM. Progesterone-mediated suppression of estradiol receptors in cynomolgus macaque cervix, endometrium and oviduct during sequential estradiol-progesterone treatment. J Steroid Biochem 1985; 22: 29–37.PubMedCrossRefGoogle Scholar
  17. 17.
    Greene GL, Press MF. Structure and dynamics of the estrogen receptor. J Steroid Biochem 1986; 24: 1–7.PubMedCrossRefGoogle Scholar
  18. 18.
    Bartelmez GW. Cyclic changes in the endometrium of the rhesus monkey (Macaca mulatta). Contrib Embryol 1951; 34: 99–144.Google Scholar
  19. 19.
    Ferenczy A, Bertrand G, Gelfand MM. Studies on the cytodynamics of human endometrial regeneration III. In vitro short-term incubation historadioautography. Am J Obstet Gynecol 1979; 134: 297–304.PubMedGoogle Scholar
  20. 20.
    West NB, Brenner RM. Estrogen receptor levels in the oviducts and endometria of cynomolgus macaques during the menstrual cycle. Biol Reprod 1983; 29: 1303–12.PubMedCrossRefGoogle Scholar
  21. 21.
    Brenner RM, Carlisle KS, Hess DL, Sandow BA, West NB. Morphology of the oviducts and endometria of cynomolgus macaques during the menstrual cycle. Biol Reprod 1983; 29: 1289–302.PubMedCrossRefGoogle Scholar
  22. 22.
    Enami J, Enami S, Koga M. Growth of normal and neoplastic mouse mammary epithelial cells in primary culture: stimulation by conditioned medium from mouse mammary fibroblasts. Gann 1983; 74: 84553.Google Scholar
  23. 23.
    McGrath CM. Augmentation of the response of normal mammary epithelial cells to estradiol by mammary stroma. Cancer Res 1984; 43: 1355–60.Google Scholar
  24. 24.
    Ferenczy A. Studies on the cytodynamics of experimental endometrial regeneration in the rabbit. Historadioautography and ultrastructure. Am J Obstet Gynecol 1977; 128: 536–45.PubMedGoogle Scholar
  25. 25.
    Cunha GR, Bigsby RM, Cooke PS, Sugimura Y. Stromal-epithelial interactions in adult organs. Cell Differ 1985; 17: 137–48.PubMedCrossRefGoogle Scholar
  26. 26.
    Brenner RM, Resko JA, West NB. Cyclic changes in oviductal morphology and residual cytoplasmic estradiol binding capacity induced by sequential estradiol-progesterone treatment of spayed rhesus monkeys. Endocrinology 1974; 95: 1094–104.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Robert M. Brenner
    • 1
  • Maryanne C. McClellan
    • 2
  • Neal B. West
    • 1
  1. 1.Oregon Regional Primate Research CenterBeavertonUSA
  2. 2.Reed CollegePortlandUSA

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