Isolation and Sequence of the Human Glucocorticoid Receptor Gene Promoter. Cloning of the Human Androgen Receptor cDNA

  • Manjapra V. Govindan
  • Marco Burelle
  • Celine Cantin
  • Martine Devic
  • Claude Labrie
  • Fernand Labrie
  • Yves Lachance
  • Gilles Leblanc
  • Claude Lefebvre
  • Pravin Patel
  • Jacques Simard
  • Udo Stropp
Part of the Serono Symposia, USA book series (SERONOSYMP)


We have isolated the promoter region of the human glucocorticoid receptor (hGR) gene from the λEMBL-3 human genomic library using synthetic oligonucleotides corresponding to the 5’ end of hGR cDNA. The clone was fine mapped by restriction digestion, hybridization with hGR cDNA probes and by DNA sequence analysis and found to contain an open reading frame corresponding to the hGR cDNA from amino acids 1–131 separated by a large intron from the 5’ noncoding sequences. The promoter region of the hGR has been identified by primer extension, nuclease S1 mapping using hGR mRNA from human prostatic carcinoma cells (LNCaP) and human breast tumor cells (MCF-7), by in vitro transcription using hGR-DNA fragments as template and by DNA sequence analysis. The sequence of the hGR promoter reveals that it contains neither a “TATA box” nor a “CART box” and has an extremely high “G+C” content. Gene transfer studies with hGR-CAT chimeric plasmids into COS-cells showed that the hormonal regulatory sequences of hGR is contained within a 4 kb EcoRl-XbaI fragment.


Androgen Receptor Glucocorticoid Receptor LNCaP Cell Mouse Mammary Tumor Virus Androgen Receptor mRNA 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Manjapra V. Govindan
    • 1
  • Marco Burelle
    • 1
  • Celine Cantin
    • 1
  • Martine Devic
    • 2
  • Claude Labrie
    • 1
  • Fernand Labrie
    • 1
  • Yves Lachance
    • 1
  • Gilles Leblanc
    • 1
  • Claude Lefebvre
    • 1
  • Pravin Patel
    • 3
  • Jacques Simard
    • 1
  • Udo Stropp
    • 2
  1. 1.MRC Group in Molecular EndocrinologyLavai University Medical CenterCanada
  2. 2.L.G.M.E., C.N.R.S., U-184INSERM Institut de Biochimie BiologiqueStrasbourgFrance
  3. 3.Department of Microbiology, Faculty of MedicineLaval UniversityCanada

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