Advances in Uterus Estradiol Receptor Phosphorylation on Tyrosine and Preliminary Evidences That Liver Glucocorticoid Receptor Might Be Phosphorylated on Tyrosine
Our group has been working since 1979 on the process which reversibly regulates hormone binding of the uterus estradiol receptor. The possibility that phosphorylation of proteins is responsible for the hormone binding to steroid receptors was proposed for the first time for the glucocorticoid receptor when it was observed that ATP shortage of thymocytes decreases their ability to bind hormone whereas ATP recovery parallels recovery of this binding (1). Subsequently, it was observed that hormone binding to different steroid receptors is inactivated by exogenous phosphatases and reactivated by processes requiring ATP (2–4). Unfortunately, neither in the case of glucocorticoid receptor nor in the case of other steroid receptors was it proved that the observed fluctuations of hormone binding were due to phosphorylation-dephosphorylation of either receptors or receptor-related proteins.
KeywordsGlucocorticoid Receptor Steroid Receptor Hormone Binding Receptor Preparation Antiphosphotyrosine Antibody
Unable to display preview. Download preview PDF.
- 4.Liao S, Rossini GP, Hiipakka RA, Chen C. Factors that can control the interaction of the androgen-receptor complex with the genomic structure in the rat prostate. In: Bresciani F, ed. Perspectives in steroid receptor research. 1980; 99–112.Google Scholar
- 15.Auricchio F, Migliaccio A, Castoria G, et al. Phosphorylation on tyrosine of oestradiol-178 receptor in uterus and interaction of oestradiol-178 and glucocorticoid receptors with antiphosphotyrosine antibodies. J Steroid Biochem 1987 (in press).Google Scholar
- 17.Auricchio F, Migliaccio A, Di Domenico M, Nola E. Oestradiol stimulates phosphorylation of its own receptor in a cell-free system. EMBO J 1987 (in press).Google Scholar
- 29a.Singh VB, Moudgil VK. Protein kinase activity of purified rat liver glucocorticoid receptor. Biochem Biophys Res Commun 1984; 1067–73.Google Scholar
- 33.Horwitz KB, McGuire WL. Nuclear mechanism of estrogen action: effect of estradiol and anti-estrogens on estrogen receptors and nuclear receptor processing. J Biol Chem 1978; 23: 8185–91.Google Scholar