Radioactive Monoclonal Antibodies Against Cell Surface Antigens for Labeling Leukocyte Subpopulations
The ability to follow the kinetics and migration of various leukocyte populations, particularly with the oxine and tropolone lipophilic chelates of In-111, has contributed greatly to our expanding knowledge of cellular immunology in recent years (1, 2, 3, 4). In the cellular immune system, lymph nodes collect and process antigen from extracellular fluid — the peripheral nodes for superficial tissues and the spleen for blood-borne antigens. The gastrointestinal tract has its own lymphoid organs for processing ingested antigens — Peyer’s patches, appendix, tonsils and adenoids (5). Labeled T cells migrate preferentially to peripheral lymph nodes and B cells to the spleen and Peyer’s patches (6). This organ-specific homing is controlled by interaction between recirculating lymphocytes and endothelial cells of post-capillary high endothelial venules (HEV) through specific surface receptors, thereby directing the cells into the lymphoid organs. A greater localization of cytotoxic T cells than helper T cells has been observed in Peyer’s patches, but their localization is equal in peripheral nodes (7).
KeywordsLabel Yield Mononuclear Leukocyte Large Granular Lymphocyte High Endothelial Venule Selective Label
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