Image Subtraction Techniques in Immunoscintigraphy

  • Jean Claude Liehn
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 152)


This paper is devoted to the application of the Image Subtraction Technique to immunoscintigraphy. It is technical, because, although based on simple principles, this method raises problems which are not always satisfactorily solved. It is divided into three parts.


Blood Pool Difference Image Scatter Diagram Label Antibody Image Subtraction 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Begent, R. H., Green, A. J., Bagshawe, K. D., Jones, B. E., Keep, P. A., Searle, F., Jewkes, R. F., Barratt, G. M., RYMAN , B. E., 1982, Liposomally entrapped second antibody improves tumour imaging with radiolabelled (first) antitumour antibody, The Lancet, October 2:739–742CrossRefGoogle Scholar
  2. Beihn, R. M., Damron J. R., Hafner T., 1974, Subtraction technique for the detection of subphrenic abscesses using 67 Ga and Tc-99m, J Nucl Med, 15:371–373PubMedGoogle Scholar
  3. Carrol, M. J., Flatman, W. D., Nimmon, C. C., Granowska, M., Britton, K. E., 1984, Congruent image registration as a pre-requisite for detecting sequential changes during radioimmunoscintigraphy. Nucl Med Commun, 5:230 (Abs.)Google Scholar
  4. Davies, J. O., Davies, E. R., Howe, K. Jackson, P. C., Pitcher, E. M., Sadowski, C. S., Stirrat, G. M., Sunderland, C. A., 1985, Radionuclide imaging of ovarian tumour with I-123-labelled monoclonal antibody (NDOG2) directed against placental alkaline phosphatase, Brit J Obstetrics and Gynaecology, 92:277–286CrossRefGoogle Scholar
  5. Deland, F. H., Kim, E. E., Simmons, G., and Goldenberg, D. M., 1980, Imaging Approach in Radioimmunodetection, Cancer Research, 40:3046–3049PubMedGoogle Scholar
  6. Fairweather, D. S., Bradwell, A. R., Dykes, P. W., Vaughan, A. T., Watson-James, S. F., Chandler, S., 1983, Improved tumour localization using indium-111 labelled antibodies, Brit Med J, 287:167–170CrossRefGoogle Scholar
  7. Ferlin, G., Borsato, N., Camerani M., Conte, R, Zotti, D., 1983, New perspectives in localizing enlarged parathyroids by Technetium-Thallium subtraction scan, J Nucl Med, 24:438–441PubMedGoogle Scholar
  8. Goldenberg, D. M., Kim, E. E., Deland, F. H., Bennett, S., and Primus F. J., 1980, Radioimmunodetection of cancer with radioactive antibodies to carcinoembryonic antigen, Cancer Research, 40:2984–2992PubMedGoogle Scholar
  9. Goldenberg, D. M., Deland, F., Kim, E., Bennett, S., Primus, F. J., van Nagell, J. R., Estes, N., DeSimone, P., Rayburn, P., 1978, Use of radiolabeled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancers by external photoscanning, New Engl J Med, 298, 1384–1388PubMedCrossRefGoogle Scholar
  10. Goodwin, D., Meares, C., Diamanti, C., McCall, M., Lai, C., Torti, F., McTigue, M. , and Martin B., 1984, Use of specific antibody for rapid clearance of circulating blood background from radiolabeled tumor imaging proteins, Eur J Nucl Med, 9:209–215PubMedCrossRefGoogle Scholar
  11. Granowska, M., Shepherd, J., Britton, K. E., Ward, B., Mather, S., Taylor-Papadimitriou, J., Epenetos, A. A., Carroll, M. J., Nimmon, C. C., Hawkins, L. A., Slevin, M., Flatman, W., Home, T., Burchell, J., Durbin, H., and Bodmer, W., 1984, Ovarian cancer: diagnosis using I-123 monoclonal antibody in comparison with surgical findings, Nucl Med Commun, 5:485–499PubMedCrossRefGoogle Scholar
  12. Granowska, M., Britton, K. E., and Shepherd, J., 1984, The detection of ovarian cancer using I-123 monoclonal antibody, Radiobiol Radiother, 25:153–160Google Scholar
  13. Green, A. J., Begent, R. H., Keep, P. A., and Bagshawe K. D., 1984, Analysis of radioimmunodetection of tumors by the subtraction technique, J Nucl Med, 25:96–100PubMedGoogle Scholar
  14. Himmelblau, D. M., 1972, Applied nonlinear programming. New York, McGraw Hill.Google Scholar
  15. Jackson, P. C., Pitcher, E. M., Davies, J. O., Davies, E. R., Sadowski, C. S., Staddon, G. E., Stirrat, G. M., and Sunderland, C. A., 1985, Radionuclide imaging of ovarian tumours with a radiolabelled (I-123) Monoclonal antibody (NDOG2), Eur J Nucl Med, 11:22–28PubMedCrossRefGoogle Scholar
  16. Liehn, J.C., Venot, A., Lebruchec, J. F., 1986, A new approach to double tracer studies. in: S.L. Bacharach (Ed.), Information Processing in Medical imaging, Martinus-Nijhoff, Dordrecht, pp. 280–297Google Scholar
  17. Mach, J. P., Buchegger, F., Forni, M. , Ritschard, J., Berche, C., Lumbroso, J.D., Schreyer, J., Girardet, C., Accolla, R. S., and Carrel, S., 1981, Use of radiolabelled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photoscanning and tomoscintigraphy, Immunology Today, 239–249Google Scholar
  18. Mach, J. P., Carrel, S., Forni, M., Ritschard, J., Donath, A., and Alberto, P., 1980, Tumor localization of radiolabeled antibodies against carcinoembryonic antigen in patients with carcinoma: a critical evaluation, New Engl J Med, 303:5–10PubMedCrossRefGoogle Scholar
  19. Nimmon, C. C., Carroll, M. J., Flatman, W., Marsden, P., Granowska, M., Home, T., Britton, K. E., 1984, Spatial probability mapping of temporal change: application to gamma camera quality control and immunoscintigraphy, Nucl Med Commun, 5:231 (Abs.)Google Scholar
  20. Ott, R. J., Grey, L. J., Zivanovic, M. A., Flower, M. A., and Trott, N. G., Moshakis, V., Coombes, R.C., Neville A. M., Ormerod, M.G., Westwood, J.H., McCready V. R. 1983, The limitation of the dual radionuclide subtraction technique for the external detection of tumours by radioiodine-labelled antibodies, Br J Radiol, 56:101–108PubMedCrossRefGoogle Scholar
  21. Overton, T.R., Heslip, P.G., Barrow, P.A., Jelinek J., 1971, Dual-radioisotope techniques and digital image-subtraction methods in pancreas visualization, J Nucl Med 12:493–498PubMedGoogle Scholar
  22. Perkins, A. C., and Pimm, M. V., 1985, Differences in tumour and normal tissue concentrations of iodine- and indium-labelled monoclonal antibody I, Eur J Nucl Med, 11:295–299PubMedCrossRefGoogle Scholar
  23. Perkins, A. C., Whalley, D. R., Hardy, J. G., 1984, Physical approach for the reduction of dual radionuclide image subtraction artifacts in immunoscintigraphy, Nucl Med Commun, 5:501–512PubMedCrossRefGoogle Scholar
  24. Pimm, M. V., Perkins, A. C., Baldwin R. W., 1985, Differences in tumour and normal tissue concentrations of iodine- and indium-labeled monoclonal antibody II, Eur J Nucl Med, 11:300–304PubMedCrossRefGoogle Scholar
  25. Rainsbury, R. M., Westwood, J. H., Coombes, R. C., Neville, A. M., Ott, R. J., Kalirai, T. S., McCready, V. R., Gazet, J-C., 1983, Location of metastatic breast carcinoma by a monoclonal antibody chelate labelled with indium-111, The Lancet, October, 22:934–938CrossRefGoogle Scholar
  26. Rankin, E. M., McVie, J. G., 1983, Radioimmunodetection of cancer: problems and potential, Brit Med J, 287:1402–1404CrossRefGoogle Scholar
  27. Shepherd, P. S., Lazarus, C. R., Mistry, R. D., and Maisey, M. N., 1985, Detection of thyroid tumour using a monoclonal I-123 anti-human thyroglobulin antibody, Eur J Nucl Med, 10:291–295PubMedCrossRefGoogle Scholar
  28. Skretting, A., 1975, An iterative Computer Algorithm for Optimization of Radionuclide Subtraction Studies, Phys. Med. Biol, 20:578–592PubMedCrossRefGoogle Scholar
  29. Sullivan, D C., Silva, J. S., Cox, CE., Haagensen, D. E., Harris, C. C., Briner, W. H., and Wells, S. A., 1982, Localization of I-131-labeled goat and primate anti-cacinoembryonic antigen (CEA) antibodies in patients with cancer, Investigative Radiology, 17:350–355PubMedCrossRefGoogle Scholar
  30. Svedlow, M., McGillem, C. D., Anuta P. E., 1978, Image Registration: Similarity Measure and Preprocessing Method Comparisons. IEEE Transactions on Aerospace and Electronic Systems, AES-14:141–149CrossRefGoogle Scholar
  31. Venot, A., Lebruchec, J. F., Golmard J. L., Roucayrol, J. C., 1983, An automated method for the normalization of scintigraphic images, J Nucl Med, 24:412–422Google Scholar
  32. Venot, A., Lebruchec, J. F., Roucayrol, J. C., 1984, A new class of similarity measures for robust image registration. Computer Vision, Graphics and Image Processing, 28:176–184CrossRefGoogle Scholar
  33. Venot, A., Golmard, J.L., Lebruchec, J.F., Pronzato, L., Walter, E., Fril, G., Roucayrol, J.C., 1984, Digital methods for change detection in medical images. in: Deconinck F. (Ed), Information Processing in Medical Imaging, Martinus Nijhoff, The Hague, 1-16.CrossRefGoogle Scholar
  34. Venot, A., Leclerc, V., 1984, Automated correction of patient motion and gray values prior to subtraction in digitized Angiography. IEEE Trans Med Imag MI3:179–186.CrossRefGoogle Scholar
  35. Venot, A., Liehn, J. C., Lebruchec, J. F., Roucayrol, J. C., 1986, The automated comparison of scintigraphic images, J Nucl Med (to be published)Google Scholar
  36. Wahl, R. L., Tuscan, M. C., Botti, J. M., 1986, Dynamic variable background subtraction: a simple means of displaying radiolabeled monoclonal antibody scintigrams, J Nucl Med, 27:545–548.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Jean Claude Liehn
    • 1
  1. 1.Institut Jean GodinotReimsFrance

Personalised recommendations