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Optimization of Mouse IgG Fragmentation Technique in the Selection of Monoclonal Antibodies for Myocardial Infarct Imaging

  • Catherine Larue
  • Corinne Chateigner
  • Patrick Gautier
  • Jacques Planchenault
  • Jocelyne Leger
Part of the NATO ASI Series book series (NSSA, volume 152)

Abstract

Irreversible ischemic injury of cardiac myocytes in infarction results in loss of cell membrane integrity, leakage of intracellular enzymes, and release of myosin fragments. Three monoclonal antibodies selected for their high affinity for human ventricular myosin heavy chains (Leger et al., 1985) are chosen for their cross-reactivity (95%) with canine myosin.

Keywords

Experimental Myocardial Infarction Necrotic Myocardium Peptic Digestion Sepharose Affinity Chromatography Myocardial Infarct Event 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Catherine Larue
    • 1
  • Corinne Chateigner
    • 1
  • Patrick Gautier
    • 1
  • Jacques Planchenault
    • 1
  • Jocelyne Leger
    • 1
    • 2
  1. 1.Centre de Recherche CLIN-MIDYMontpellierFrance
  2. 2.Faculte de PharmacieINSERM U300MontpellierFrance

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