Evidence for Contrasuppression in Patients with Crohn’s Disease

  • A. Raedler
  • S. Schreiber
  • A. de Weerth
  • B. Brinkmann
  • K. Sandgren
  • H. G. Thiele
  • H. Greten
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


Vicia villosa agglutinin (WA) is a lectin that binds specifically to glycoconjugates with terminal N-acetyl-galactose. Originally murine T cells binding to WA are reported to be involved in cytotoxicity (l) however this has been contradicted by others (2,3). Moreover murine and human T cells reacting with WA were suggested to be involved in contrasuppression (4–7). T cells displaying contrasuppressor activity inhibit the action of suppressor cells on helper cells, thus enhancing immune response. Beside binding sites for VVA, contrasuppressor T cells in mice express Lyt-2(corresponding to CD8, contrasuppressor inducer), Lyt-1 (corresponding to CD4, contrasuppressor effector) or both determinants (contrasuppressor acceptor or transducer cells). Additionally I-J determinants are exhibited that are however different from those found on feedback suppressor cells (8).It has been suggested that contrasuppression is responsible for regulation of local immune response in particular in the gut mucosa (5). Alterations in contrasuppression have further been suspected to be involved in autoimmunity (9)f although experimental data to support this thesis are sparse (10).


Ulcerative Colitis Local Immune Response Helper Activity Inflame Mucosa Strategic Defense Initiative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    A. Kimura, H. Wigzell, G. Holmquist, B. Ersson and P. Carlson, Selective affinity fractionation of murine cytotoxic lymphocytes (CTL), J. exp. Med. 149; 473 (1979).PubMedCrossRefGoogle Scholar
  2. 2.
    V. I. Braciale, H. P. Friedman and T. J. Braciale, A method for the preparation of Vicia villosa lectin and rosette procedure Tor fractionation of lectin binding lymphocytes, J.Immunol.Meth. 43: 241 (1981).CrossRefGoogle Scholar
  3. 3.
    L. Lang, J. P. Banga, A. M. Varey, H. Gunn, A. Cooke and I. M. Roitt, Direct staining of mouse T lympho-blasts with fluoresceinated Vicia villosa lectin, Immunol. 46: 769 (1982).Google Scholar
  4. 4.
    R. K. Gershon, D. D. Eardley, S. Durum, D. R. Green F. W. Shen, K. Yamauchi, H. Cantor and D. B. Murphy, Contrasuppression, a novel immunoregulatory activity, J. exp. Med. 153: 1533–46 (1981).PubMedCrossRefGoogle Scholar
  5. 5.
    D. R. Green, J. Gold, S. St.Martin and R. Gershon, Microenvironmental immunoregulation: possible role of contrasuppressor cells in maintaining immune responses in gut-associated lymphoid tissue, Proc.Natl.Acad., Sci. USA, 79: 889–92 (1982).CrossRefGoogle Scholar
  6. 6.
    T. Lehner, J. Avery and T. Jones, Separation and Characterisation of a subset of human T8 cells which function as antigen presenting and contrasuppressor cells, Immunol. 54: 713–22 (1985).Google Scholar
  7. 7.
    T. Lehner, J. Avery and T. Jones, The role of a human antigen specific T8 cell subset in antigen presentation, helper function and contrasuppression, Clin, exp.Immunol. 61: 203–213 (1985).Google Scholar
  8. 8.
    K. Yamauchi, M. Taniguchi, D. Green and R. K. Gershon, The use of a monoclonal I-J-specific antibody to distinguish cells in the feedback suppression circuit from those in the contrasuppressor circuit, Immuno-genetics 16: 551–58 (1982).Google Scholar
  9. 9.
    T. Lehner, Antigenpresenting contrasuppressor human T cells, Immunol. Today 7: 87–92 (1986).Google Scholar
  10. 10.
    D. R. Green, Immunoregulation by contrasuppressor cells, Eos 4, 3: 155–61 (1983).Google Scholar
  11. 11.
    A. Raedler, H. J. Lenz, K. Sandgren, A. de Weerth, K. H. Schulz, H. G. Thiele and H. Greten, Phenotype and functional properties of Vicia villosa agglutinin (WA) binding T cells in patients with Crohn’s disease, Submitted.Google Scholar
  12. 12.
    C. D. Elson, M. F. Kagnoff, C. Fiocchi, A. D. Befus and S. Targan, Intestinal immunity and inflammation: Recent progress, Gastroenterology 91: 746–68 (1986).PubMedGoogle Scholar
  13. 13.
    C. D. Elson, E. Machelski, D. B. Weiserbs, T cell B cell regulation in the intestinal lamina propria in Crohn’s disease, Gastroenterology 89: 321–27 (1985).PubMedGoogle Scholar
  14. 14.
    B. J. Underdown and J. M. Schiff, Immunoglobulin A: Strategic defense initiative at the mucosal surface, Ann.Rev.Immunol. 4: 389–417 (1986).CrossRefGoogle Scholar
  15. 15.
    K. Baklien and P. Brandzaeg, Comparative mapping of the local distribution of immunoglobulin-containing cells in ulcerative colitis and Crohn’s disease of the colon, Clin. exp. Immunol. 22: 197 (1975).PubMedGoogle Scholar
  16. 16.
    D. F. Keren, H. Appelman, W.D. Dobbins, J. J. Wells, B. Whisenant, J. Foley, R. Dieterle and K. Geisengra, Correlation of histopathologic evidence of the disease activity with the presence of immunoglobulin-containing cells in the colon of patients with inflammatory bowel disease, Hum.Pathol. 15: 757 (1984).PubMedCrossRefGoogle Scholar
  17. 17.
    R. P. MacDermott, G. S. Nash, M. J. Bertovich, M. V. Seiden, M. J. Bragdon and M. G. Beale, Activation of IgM, IgG and IgA synthesis and secretion by peripheral blood and intestinal mononuclear cells from patients with ulcerative colitis and Crohn’s disease, Gastroenterology 81: 844 (1981).PubMedGoogle Scholar
  18. 18.
    R. P. MacDermott, M. G. Scott, G. S. Nash, K. Macke, M. J. Bertovich and M. H. Nahm, Increased spontaneous synthesis and secretion of immunoglobulin G subclass 1 (lgG1) by ulcerative colitis intestinal mononuclear cells, Gastroenterology 88: 1483 (1985).Google Scholar
  19. 19.
    A. Raedler, S. Fraenkel, G. Klose, K. Seyfarth and H. G. Thiele, Involvement of the immune system in the pathogenesis of Crohn’s disease. Expression of the T9 antigen on the peripheral immunocytes correlates with the severity of the disease, Gastroenterology 88: 978–83 (1985).PubMedGoogle Scholar
  20. 20.
    A. Raedler, S. Fraenkel, G. Klose and H. G. Thiele, Elevated numbers of peripheral T cells in inflammatory bowel diseases displaying T9 antigen and Fc-alpha receptors, Clin. exp. Immunol. 60: 518–24 (1985).PubMedGoogle Scholar
  21. 21.
    W. H. Kutteh, W. J. Koopman, M. E. Conley, M. L. Egnan and J. Mestecky, Production of predominately IgA by human peripheral blood lymphocytes stimulated in vitro with mitogens, J. exp. Med. 152: 1424–29 (1980).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • A. Raedler
    • 1
  • S. Schreiber
    • 1
  • A. de Weerth
    • 1
  • B. Brinkmann
    • 1
  • K. Sandgren
    • 1
  • H. G. Thiele
    • 1
  • H. Greten
    • 1
  1. 1.Med. Dept. UnivHamburg 20Germany

Personalised recommendations