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A Monoclonal Antibody which Reacts Specifically with a Population of Bovine Lymphocytes Lacking B cell and T cell Markers

  • W. I. Morrison
  • N. D. MacHugh
  • A. Bensaid
  • B. M. Goddeeris
  • A. J. Teale
  • D. J. McKeever
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)

Abstract

In the last few years a number of monoclonal antibodies (MAb) specific for bovine T lymphocytes has been produced. These include antibodies which react with the sheep erythrocyte receptor (designated BoT2) (Davis et al., in press) and thus can be used as pan-T cell markers, and antibodies to the two major subset markers BoT4 and B0T8 (Baldwin et al., 1986; Ellis et al., 1986). When these antibodies are used, in conjunction with reagents that identify B lymphocytes and monocytes, to phenotype bovine leukocytes, there is a residual population of lymphocytes comprising 10–20% of peripheral blood mononuclear cells (PBM) that is unreactive with these reagents. Recently, we have produced a MAb that reacts with a proportion of these “null” lymphocytes. Herein, we will summarise our findings with this antibody.

Keywords

Peripheral Blood Mononuclear Cell Secondary Lymphoid Tissue Peripheral Blood Mononuclear Human Leukocyte Differentiation Antigen Major Histocompatibility Complex Restriction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • W. I. Morrison
    • 1
  • N. D. MacHugh
    • 1
  • A. Bensaid
    • 1
  • B. M. Goddeeris
    • 1
  • A. J. Teale
    • 1
  • D. J. McKeever
    • 1
  1. 1.International Laboratory for Research on Animal DiseasesNairobiKenya

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