Xenogeneic Lymphocyte Migration in the Foetal Lamb and Unsuckled Neonatal Piglet: Evidence for the Evolutionary Conservation and Heterogeneity of Migration Determinants
Lymphocyte recirculation provides the means of rapidly bringing the pool of antigen-reactive cells to specialized sites of antigen presentation in organised lymphoid tissues. Entry into these is via postcapillary venules (PCV) with high endothelium (HEV)(1) and evidence is emerging of differences in the HEV determinants and lymphocyte receptors involved in this traffic, for instance into Peyer’s patches (PP), surface and mesenteric lymph nodes (LN) and chronic inflammatory tissue(l,2,3). This ‘active’ entry is reversibly inhibited by trypsin-pretreatment of the lymphocytes, distinguishing it from the passive arrival of lymphocytes in spleen, bone marrow, liver, lung and other non lymphoid tissues(l). Recent detailed studies have charted the migration behaviour of lymphocytes in the young pig and revealed evidence of active entry and heterogeneity of lymphocyte migration into the surface and visceral LN, jejunal, ileal and colonic PP, tonsils and tongue papillae and thymus(4,5,6). The unusual structural inversion and the lack of lymphocytes in the draining lymph of LNs(7) led to the finding that lymphocyte traffic both in and out of LNs is via PCVs(5,6,7). This communication uses the transfer of labelled xenogeneic lymphocytes into foetal lambs and unsuckled neonatal piglets, which are immunologically virgin and lack natural, antibodies and NK cell activity(8), and other evidence to elucidate the HEV heterogeneity and unusual two-way lymphocyte traffic through PCVs in the pig.
KeywordsNewborn Piglet Foetal Lamb Active Entry Thoracic Duct Lymph Organise Lymphoid Tissue
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