Xenogeneic Lymphocyte Migration in the Foetal Lamb and Unsuckled Neonatal Piglet: Evidence for the Evolutionary Conservation and Heterogeneity of Migration Determinants

  • R. M. Binns
  • S. T. Licence
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


Lymphocyte recirculation provides the means of rapidly bringing the pool of antigen-reactive cells to specialized sites of antigen presentation in organised lymphoid tissues. Entry into these is via postcapillary venules (PCV) with high endothelium (HEV)(1) and evidence is emerging of differences in the HEV determinants and lymphocyte receptors involved in this traffic, for instance into Peyer’s patches (PP), surface and mesenteric lymph nodes (LN) and chronic inflammatory tissue(l,2,3). This ‘active’ entry is reversibly inhibited by trypsin-pretreatment of the lymphocytes, distinguishing it from the passive arrival of lymphocytes in spleen, bone marrow, liver, lung and other non lymphoid tissues(l). Recent detailed studies have charted the migration behaviour of lymphocytes in the young pig and revealed evidence of active entry and heterogeneity of lymphocyte migration into the surface and visceral LN, jejunal, ileal and colonic PP, tonsils and tongue papillae and thymus(4,5,6). The unusual structural inversion and the lack of lymphocytes in the draining lymph of LNs(7) led to the finding that lymphocyte traffic both in and out of LNs is via PCVs(5,6,7). This communication uses the transfer of labelled xenogeneic lymphocytes into foetal lambs and unsuckled neonatal piglets, which are immunologically virgin and lack natural, antibodies and NK cell activity(8), and other evidence to elucidate the HEV heterogeneity and unusual two-way lymphocyte traffic through PCVs in the pig.


Newborn Piglet Foetal Lamb Active Entry Thoracic Duct Lymph Organise Lymphoid Tissue 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Woodruff J.J., Clarke L.M. & Chin Y.H. Ann. Rev. Immunol. 5, 201 (1987).CrossRefGoogle Scholar
  2. 2.
    Jalkanen E., Steere A.C., Fox R.I. & Butcher E.C. Science 233, 556 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    Chin W. & Hay J.B. Gastroenterology 79, 1231 (1980).PubMedGoogle Scholar
  4. 4.
    Binns R.M. & Licence S.T. Microenvironments in the lymphoid system p.661, Plenum NY (1985).Google Scholar
  5. 5.
    Binns R.M., Pabst R. & Licence S.T. Swine in Biomedical Research, Vol. III p.1837 Plenum NY (1986).Google Scholar
  6. 6.
    Binns R.M. & Pabst R. Migration and homing of lymphoid cells. Vol. II Ch. 16, CRC Press. (In press)Google Scholar
  7. 7.
    Binns R.M. Vet. Immunol. Immunopath. 3, 95 (1982).CrossRefGoogle Scholar
  8. 8.
    Charley B., Petit E. & Bonnardiere C.La. Ann. Recherche Vet. 16, 399 (1985).Google Scholar
  9. 9.
    Pearson, L.D., Simpson-Morgan M.W. & Morris B. J. exp. Med. 143, 167 (1976).PubMedCrossRefGoogle Scholar
  10. 10.
    Binns R.M. J. Immunol. Meth. 21, 197 (1978).CrossRefGoogle Scholar
  11. 11.
    Pabst R., Binns R.M. & Licence S.T. Immunology 56, 301 (1985).PubMedGoogle Scholar
  12. 12.
    Pabst R. Migration and homing of lymaphoid cells. Vol. I. CRC Press (in press).Google Scholar
  13. 13.
    Pabst R., Binns R.M., Peter M. and Licence, S.T. This proceedings.Google Scholar
  14. 14.
    Rannie G.H., Smith M.E. & Ford W.L. Nature 267, 520 (1977).CrossRefGoogle Scholar
  15. 15.
    Young J.Z. The life of vertebrates. Oxford univ. Press (1962)Google Scholar
  16. 16.
    Rolstad B. & Ford W.L. Immunol. Rev. 73, 87 (1986).CrossRefGoogle Scholar
  17. 17.
    Binns R.M. & Licence S.T. Immunology, 49, 727 (1983).PubMedGoogle Scholar
  18. 18.
    Butcher E.C. Scollay R. & Weissman I. Nature, 280, 496 (1979).PubMedCrossRefGoogle Scholar
  19. 19.
    Biggs P.M. Acta Anat. 29, 36 (1957).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • R. M. Binns
    • 1
  • S. T. Licence
    • 1
  1. 1.Immunology DepartmentAFRC Institute of Animal Physiology and Genetic ResearchCambridgeUK

Personalised recommendations