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Rat Immunoglobulin Genes have Comparable Patterns of JH Rearrangement in Normal Peripheral B Cells and In Pre-B And Cultured TdT+ Cells from Bone Marrow

  • S. V. Hunt
  • E. S. Medlock
  • D. L. Greiner
  • I. Goldschneider
  • D. Opstelten
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)

Summary

Pre-B cells from normal rat bone marrow were isolated by first rosettedepleting granulocytes, T cells and stem cells with W3/13 mAb and mature B cells with anti-Ig mAb, and then in some samples enriching by FACS-sorting HIS24+ cells. Presumptive precursors of these pre-B cells possessing terminal deoxy-nucleotidyl transferase were prepared by culture of normal marrow. The extent of rearrangement of kappa and heavy chain genes was estimated on both these types of cells and on mature peripheral B cells by measuring the disappearance of the germ-line restriction fragments encoding J segments (other than JH1) relative to a non-rearranging CK fragment. Pre-B cells and B cells showed similar degrees of rearrangement of their kappa genes, leaving roughly half in germ-line form; the TdT+-enriched cells showed approximately similar heavy chain rearrangement as the peripheral B cells. Some non-germ-line restriction fragments hybridising with JH in sufficient amount to register as discrete bands on Southern blots were found in common between these different cell populations taken from different individual donors. These may represent preferred D-JH or perhaps V-D-JH rearrangement arising repetitively in independent clones, or may be due to dominance by a few preferred clones.

Keywords

Heavy Chain Gene Normal Marrow Adherent Fraction Kappa Gene Presumptive Precursor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • S. V. Hunt
    • 1
  • E. S. Medlock
    • 2
  • D. L. Greiner
    • 2
  • I. Goldschneider
    • 2
  • D. Opstelten
    • 3
    • 4
  1. 1.Sir William Dunn School of PathologyUniversity of OxfordOxfordUK
  2. 2.Department of PathologyUniversity of Connecticut Health CenterFarmingtonUSA
  3. 3.Histologisch LaboratoriumRijksuniversiteit GroningenThe Netherlands
  4. 4.Dutch Foundation for Medical and Health Research MEDIGONUSA

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