Establishment of Lymph Node Derived Endothelial Cell Lines Which Show Lymphoid Cell Binding
The recirculation of lymphocytes through distinct parts of the body is regulated at least partially by sets of homing receptors on lymphocytes recognizing organ specific determinants on high endothelial venules (HEV). In the mouse, interaction between lymphocytes and peripheral node HEV can be inhibited by the monoclonal antibody MEL-14, reacting with a ubiquitinated 90 kD glycoprotein on the lymphocyte surface (Ref.1,2). Recently monoclonal antibodies against putative ligands for lymphatic receptors on endothelial cells have been obtained (Ref.3), and an HEV-specific marker, defined by the monoclonal antibody MECA 325, has been described (Ref.4). However, molecular analysis of such molecules is complicated by the limited availability of endothelial cells carrying homing receptor ligands. Attempts to bring these specialised endothelial cells into culture succeeded eventually in the rat system (Ref.5), but not in the mouse. Prerequisite for a molecular characterisation is a source with larger quantities of endothelial cells than those from the lymphatic organs of the mouse. To achieve this we tried to transform high endothelial cells to establish permanently growing endothelial cell lines of mouse origin.
KeywordsEndothelial Cell Endothelial Cell Line Transform Cell Line Peripheral Lymph Node High Endothelial Venule
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