Advertisement

Establishment of Lymph Node Derived Endothelial Cell Lines Which Show Lymphoid Cell Binding

  • Reiner Harder
  • Birgit Schütt
  • Adrian Duijvestijn
  • Heinz-Günter Thiele
  • Alf Hamann
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)

Abstract

The recirculation of lymphocytes through distinct parts of the body is regulated at least partially by sets of homing receptors on lymphocytes recognizing organ specific determinants on high endothelial venules (HEV). In the mouse, interaction between lymphocytes and peripheral node HEV can be inhibited by the monoclonal antibody MEL-14, reacting with a ubiquitinated 90 kD glycoprotein on the lymphocyte surface (Ref.1,2). Recently monoclonal antibodies against putative ligands for lymphatic receptors on endothelial cells have been obtained (Ref.3), and an HEV-specific marker, defined by the monoclonal antibody MECA 325, has been described (Ref.4). However, molecular analysis of such molecules is complicated by the limited availability of endothelial cells carrying homing receptor ligands. Attempts to bring these specialised endothelial cells into culture succeeded eventually in the rat system (Ref.5), but not in the mouse. Prerequisite for a molecular characterisation is a source with larger quantities of endothelial cells than those from the lymphatic organs of the mouse. To achieve this we tried to transform high endothelial cells to establish permanently growing endothelial cell lines of mouse origin.

Keywords

Endothelial Cell Endothelial Cell Line Transform Cell Line Peripheral Lymph Node High Endothelial Venule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gallatin, W.M., I.L. Weissman and E.C. Butcher. 1983. A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature 303: 30.CrossRefGoogle Scholar
  2. 2.
    St. John, T. W.M. Gallatin, M. Siegelman, H.T. Smith, V.A. Fried and I.L. Weissman. 1986. Expression cloning of a homing receptor cDNA: Ubiquitin is the reactive species. Science 231: 845.PubMedCrossRefGoogle Scholar
  3. 3.
    Streeter, P., E. Lakey, B.T. Rouse, R. Bargatze and E.C. Butcher. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Submitted.Google Scholar
  4. 4.
    Duijvestijn, A.M., M. Kerkhove, R.F. Bargatze and E.C. Butcher. 1987. Lymphoid tissue-and inflammation-specific endothelial cell differentiation defined by monoclonal antibodies. J. Immunol. 138: 713.PubMedGoogle Scholar
  5. 5.
    Ager, A. 1987. Isolation and culture of high endothelial cells from rat lymph nodes. J. Cell Science 87: 133.PubMedGoogle Scholar
  6. 6.
    Somers, S.D., C.C. Whisnant and D.O. Adams. 1986. Quantification of the strength of cell-cell adhesion: The capture of tumor cells by activated murine macrophages proceeds through two distinct stages. J. Immunol. 136: 1490.PubMedGoogle Scholar
  7. 7.
    Pierres, M., C. Goridis, and P. Golstein. 1982. Inhibition of murine T cell-mediated cytolysis and T cell proliferation by a rat monoclonal antibody immunoprecipitating two lymphoid cell surface polypeptides of 94000 and 180000 molecular weight. Europ. J. Immunol. 12: 60.CrossRefGoogle Scholar
  8. 8.
    Kaufmann, S.H.E., K. Eichmann, I. Müller and L. Wrazel. 1985. Vaccination against the intracellular bacterium Listeria monocygotes with a clonotypic antiserum. J. Immunol. 134: 4123.PubMedGoogle Scholar
  9. 9.
    Woodruff, J.J., I.M. Katz, L.E. Lucas and H.B. Stamper, Jr. 1977. An in vitro model of lymphocyte homing. II.:Membrane and cytoplasmic events involved in lymphocyte adherence to specialized high-endothelial venules of lymph nodes. J. Immunol. 119: 1603.PubMedGoogle Scholar
  10. 10.
    Chin, Y.-H., R.A. Rasmussen, A.K. Cakirhoglu and J.J. Woodruff. 1984. Lymphocyte recognition of lymph node high endothelium. VI. Evidence of distinct structures mediating binding to high endothelial cells of lymph nodes and Peyer’s patches. J. Immunol. 133: 2961.PubMedGoogle Scholar
  11. 11.
    Haskard, D., D. Cavender, P. Beatty, T. Springer and M. Ziff. 1986. T lymphocyte adhesion to endothelial cells: Mechanisms demonstrated by anti-LFA-1 monoclonal antibodies. J. Immunol. 137: 2901.PubMedGoogle Scholar
  12. 12.
    Chin, Y.-H., R.A. Rasmussen, J.J. Woodruff and T.G. Easton. 1986. A monoclonal anti-HEBFpp antibody with specificity for lymphocyte surface molecules mediating adhesion to Peyer’s patch high endothelium of the rat. J. Immunol. 136: 2556.PubMedGoogle Scholar
  13. 13.
    Rasmussen, R.A., Y.H. Chin, J.J. Woodruff and T.G. Easton. 1985. Lymphocyte recognition of lymph node high endothelium. VII. Cell surface proteins involved in adhesion defined by monoclonal anti-HEBFln (A.11) antibody. J. Immunol. 135: 19.PubMedGoogle Scholar
  14. 14.
    Jalkanen, S.T., R.F. Bargatze, L.R. Herron and E.C. Butcher: 1986. A lymphoid cell surface glycoprotein involved in endothelial cell recognition and lymphocyte homing in man. Eur. J. Immunol. 16: 1195.PubMedCrossRefGoogle Scholar
  15. 15.
    Jalkanen, S., A.C. Steere. R.I. Fox and E.C. Butcher. 1986. A distinct endothelial cell recognition system that controls lymphocyte traffic into inflamed synovium. Science 233: 556.PubMedCrossRefGoogle Scholar
  16. 16.
    Hamann, A., Jablonski-Westrich, D., Duijvestijn, A., E.C. Butcher, H. Baisch, R. Harder and H.-G. Thiele. Evidence for a accessory role of LFA-1 in lymphocyte-high endothelium interaction during homing. J. Immunol., in press.Google Scholar
  17. 17.
    Mentzner, S.J., S.J. Burakoff and D.V. Faller. 1986. Adhesion of T lymphocytes to human endothelial cells is regulated by the LFA-1 membrane molecule. J. Cell. Physiol. 126: 285.CrossRefGoogle Scholar
  18. 18.
    Hendriks, H.R., C. Korn, A.M. Duijvestijn and G. Kraal. 1987. Changes in lymphocyte binding and expression of His 22 of high endothelial venules in rat lymph nodes after occlusion of afferent lymph flow. 9th international conference on lymphatic tissues and germinal centres in immune reactions. Oslo, M-15.Google Scholar
  19. 19.
    Duijvestijn, A.M., A.B. Schreiber and E.C. Butcher. 1986. Interferonregulates an antigen specific for endothelial cells involved in lymphocyte traffic. Proc. Natl. Acad. Sci. 83: 9114.PubMedCrossRefGoogle Scholar
  20. 20.
    Horst, E., S.T. Pals, A.M. Duijvestijn, J.v. Maurik, G. Kraal, E.C. Butcher and C.J.L.M. Maijer. 1987. Expression and regulation of an antigen specific for andothelium involved in human lymphocyte homing. 9th conference on lymphativ tissues and germinal center in immune reactions. Oslo, M16.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Reiner Harder
    • 1
  • Birgit Schütt
    • 1
  • Adrian Duijvestijn
    • 1
  • Heinz-Günter Thiele
    • 1
  • Alf Hamann
    • 1
  1. 1.Abt. f. klin. Immunologie, I. Med. KlinikUniv. krankenhausGermany

Personalised recommendations