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Suppression of Immunoglobulin Production by Germinal Centre HNK-1+ CD3+ Cells

  • D. Banerjee
  • J. Baril
  • D. A. Bell
  • D. McFarlane
  • R. Karim
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)

Abstract

Germinal centre T cells co-expressing HNK-1 antigen have little lytic activity against NK targets (K562 cells). In order to determine whether these cells regulate B cell function, they were purified from human tonsils by panning on anti-HNK-1 antibody coated Petri dishes and co-cultured with autologous and allogeneic tonsillar T and B cells in the presence of pokeweed mitogen. At the end of 7 days of culture, supernatants were assayed for immunoglobulin concentration by ELISA. A dose- dependent suppression of both IgG and IgM production was demonstrated at ratios from 1:125 to 1:16 of HNK-1+ cells to B cells, but enhancement was observed at very low ratios (less than 1:500) or ratios exceeding 1:16 in some tonsil preparations. Similar results were obtained with peripheral blood HNK-1+ cells but without enhancement in some cases at the extremes of HNK-1+ cells to B cell ratios. The suppression was not MHC-restricted. These preliminary experiments indicate that germinal centre HNK-1+ cells may be intrafollicular suppressor cells.

Keywords

Natural Killer K562 Cell Helper Cell Immunoglobulin Production Pokeweed Mitogen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • D. Banerjee
    • 1
  • J. Baril
    • 1
  • D. A. Bell
    • 1
  • D. McFarlane
    • 1
  • R. Karim
    • 1
  1. 1.Department of Pathology and MedicineUniversity of Western OntarioLondonCanada

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