Mycobacterium Tuberculosis Antigen Specific Human T-Cell Lines are Cytolytic to Autologous Antigen Pulsed Macrophages
Mycobacterium tuberculosis is a bacterial pathogen capable of survival and replication within human macrophages. Current ideas about antimycobacterial immunity grew out of the pioneering work of Lurie1 and Mackaness2. In summary, it is believed that acquired resistance to tuberculosis depends on T-cell mediated macrophage activation, resulting in an enhancement of the bacteriostatic or bacteriocidal capacity of the latter cells3. However, experimental confirmation of the above using human T-cells and macrophages has been very difficult (reviewed by Rook, 1987)4. Recent evidence indicates that murine T-cells possess cytolytic capacity against mycobacterial antigen bearing macrophages5. Adoptive transfer of such L3T4 negative Lyt-2 positive cells induces protection against intracellular bacterial infections including tuberculosis5,6. Therefore, we have investigated the generation of human T-cell lines with cytotoxic capacity against mycobacterial-antigen-pulsed macrophages.
KeywordsMycobacterial Antigen Lepromatous Leprosy Cytotoxicity Experiment Tuberculoid Leprosy Macrophage Monolayer
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