Advertisement

Homing Receptor Expression in Grafts of Purified Thymic Epithelium

  • Greg A. Perry
  • David A. Crouse
  • Morris O. Dailey
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)

Abstract

Grafts of purified thymic epithelium have been used to study the role of the thymic microenvironment in T cell differentiation1–6. The ability of such grafts to immunologically reconstitute either nude mice or thymectomized, irradiated and hematopoietically reconstituted hosts has demonstrated that they are only partially able to reconstitute the host animal, as neither lymphocyte phenotype frequencies nor functional responsiveness of the graft derived peripheral (splenic) T cell population reaches normal (intact) levels2,3,6. More recently it has been suggested that the expression of lymphocyte homing receptors may be important in the selection of thymocytes destined to emigrate from the thymus, as well as their localization in the periphery7–9. In the studies reported here, we have examined homing receptor expression in grafts of purified thymic epithelium and in the peripheral lymphoid organs of grafted nude mice. Results showed that, although incomplete, T cell reconstitution did take place in these animals. The presence of MEL-14 positive cells in the graft suggests that the presence or absence of homing receptor expression in these grafts was not solely responsible for the incomplete T cell emigration and peripheralization seen in these animals.

Keywords

Nude Mouse Immunodeficient Mouse Peripheral Lymph Node Homing Receptor Peripheral Lymphoid Organ 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Jordan, R.K., A.L. Bentley, G.A. Perry and D.A. Grouse, Thymic epithelium I. Lymphoid-f ree organ cultures grafted in syngeneic intact mice. J. Immunol. 134: 2155–2160 (1985).PubMedGoogle Scholar
  2. 2.
    Crouse, D.A., G.A. Perry and R.K. Jordan, Transplantation of lymphoid free thymic epithelium in syngeneic and allogeneic immunodeficient mice. In: Regulation of the Immune System (E. Sercarz, H. Cantor and L. Chess, eds), New York: Alan R. Liss, pp. 231–242 (1984).Google Scholar
  3. 3.
    Crouse, D.A. and R.K. Jordan, Studies of adoptive transfer of thymic rudiments., In: Leukocytes and Host Defense (J.J. Oppenheim and D.M. Jacobs, eds), New York: Alan R. Liss, pp. 11–20 (1986).Google Scholar
  4. 4.
    Jenkinson, E.J., L.L Franchi, R. Kingston and J.J.T. Owen, Effect of deoxyguanosine treatment on lymphopoiesis in the developing thymus in vitro: Application in the production of chimeric thymic rudiments. Eur. J. Immunol. 12: 583–587 (1982).PubMedCrossRefGoogle Scholar
  5. 5.
    Sehuite-Wissermann, H., D. Manning and R. Hong, Transplantation of cultured thymic fragments. I. Morphologic and technical considerations. Scand. J. Immunol. 8: 387–396 (1978).CrossRefGoogle Scholar
  6. 6.
    Perry, G.A., R.K. Jordan and D.A. Crouse, Immune reconstitution in thymus grafted hematopoietic radiation chimeras. Radiat. Res. (submitted).Google Scholar
  7. 7.
    Reichert, R.A., W.M. Gallatin, E.C. Butcher and I.L. Weissman, A homing receptor-bearing cortical thymocyte subset: Implications for thymus cell migration and the nature of cortisone-resistant thymocytes. Cell 38: 89–99 (1984).PubMedCrossRefGoogle Scholar
  8. 8.
    Reichert, R.A., I.L. Weissman and E.C. Butcher, Phenotypic analysis of thymocytes that express homing receptors for peripheral lymph nodes. J. Immunol. 136: 3521–3528 (1986).PubMedGoogle Scholar
  9. 9.
    Reichert, R.A., L. Jerabek, W.M. Gallatin, E.C. Butcher and I.L. Weissman, Ontogeny of lymphocyte homing receptor expression in the mouse. J. Immunol. 136: 3535–3542 (1986).PubMedGoogle Scholar
  10. 10.
    Jordan, R.K. and D.A. Crouse, Non-lymphoid cells and intrathymic T-cell differentiation. In: Development and Differentiation of Vertebrate Lymphocytes (J.D. Horton, ed), Amsterdam: Elsevier-North Holland, pp. 47–61 (1980).Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Greg A. Perry
    • 1
  • David A. Crouse
    • 2
  • Morris O. Dailey
    • 1
  1. 1.Department of PathologyUniversity of IowaIowa CityUSA
  2. 2.Department of AnatomyUniversity of Nebraska Medical CenterOmahaUSA

Personalised recommendations