Studies on Thymic Involution: Growth Potential and DNA Repair of the Stromal Cells

  • Myra Small
  • Moshe Aronson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


Involution of the thymus is reflected in primary cultures of mouse thymic stromal cells which we have described at the previous Germinal Center Conference (1,2). When these cultures are grown from preinvolution mice, the majority of the cells arrange themselves into networks with long cytoplasmic interconnections between the cells. In cultures from older mice, cellularity is considerably reduced especially of the cells with processes. Correspondingly, in the post involution cultures an increased proportion of cells grow as confluent carpets of paving stones (1,2). The present study was carried out a) to Identify which cells each of these are and b) to use these cultures to test the hypothesis that involution, as an accelerated form of aging may result from failure to repair damaged DNA. For cell identification we used fluorescent antibody staining, and for studies of DNA repair, autoradiography of unscheduled DNA synthesis after irradiation with UV. Cortical epithelial cells turned out to be those with decreased growth in cultures from aging mice, but the cells which remain continue to repair UV-induced damage to their DNA.


Ammonium Sulfate Evans Blue Mouse Thymus Medullary Epithelial Cell Accelerate Form 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Small M, Barr-Nea L, and Aronson M. Eur. J. Immunol. 14: 936, 1984.PubMedCrossRefGoogle Scholar
  2. 2.
    Small M, Barr-Nea L, and Aronson M. in Microenvironments in the Lymphoid System GGB Klaus ed. Plenum Press N.Y. 1985, p. 273.Google Scholar
  3. 3.
    Gown AM, and Vogel AM. J. Cell Biol. 95: 414, 1982.PubMedCrossRefGoogle Scholar
  4. 4.
    van Vliet E, Melis M and van Ewijk W. Eur. J. Immunol. 14: 524, 1984.PubMedCrossRefGoogle Scholar
  5. 5.
    Rouse RV. Manuscript submitted.Google Scholar
  6. 6.
    Marcucci F, Klein B, Kirchner H, and Zawatky R. Eur. J. Immunol. 12: 787, 1982.PubMedCrossRefGoogle Scholar
  7. 7.
    Laster AJ, Itoh T, Palker TJ, and Haynes BF, Differentiation 31: 67, 1986.PubMedCrossRefGoogle Scholar
  8. 8.
    Small M, van Ewijk W, Gown AM and Rouse RV, manuscript submitted.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Myra Small
    • 1
  • Moshe Aronson
    • 1
  1. 1.Dept. of Cell Biology and Histology Sackler School of MedicineTel-Aviv UniversityRamat-AvivIsrael

Personalised recommendations