Surface IgD Phenotype of Rat Germinal Centre Precursor Cells

  • R. H. Vonderheide
  • S. V. Hunt
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


Germinal centre B cells in lymph nodes differ from most other lymph node B cells by their lack of surface IgD (1). The question arises whether germinal centre precursor cells (GCPC) are also slgD-negative or whether they express slgD but lose it as they initiate or otherwise contribute to GC formation. We investigated this question by employing the monoclonal antibody HIS22, which labels all B cells in rat lymph nodes except those in GCs (2). HIS22 and anti-kappa antibodies were detected in cell suspensions by separate fluorochromes so that germinal centre B cells (HIS22) kappa+) were distinguished from non-GC B cells (HIS22+ kappa+). Acute, non-irradiation chimeras were established by injecting B cells from TDL of one kappa allotype into host rats whose B cells expressed the opposite allotype. The relative tendency for donor cells to localise in GCs as opposed to non-GC B cell areas was measured by two-colour flow cytometry of lymph nodes sampled during a primary immune response. By using donor TDL depleted of slgD+ cells, the delta heavy chain surface phenotype of GCPC was investigated


Cervical Lymph Node Germinal Centre Donor Cell Fluorescence Activate Cell Sorting Primary Immune Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kraal, G., I.L. Veissman, and E.C. Butcher. 1982. Germinal centre B cells: antigen specificity and changes in heavy chain class expression. Nature. 298: 377.PubMedCrossRefGoogle Scholar
  2. 2.
    Kroese, F.G.M., A.S. Vubbena, D. Opstelten, G.J. Deenen, E.H. Schwander, L. De Leij, H. Vos, S. Poppema, J. Volberda, and P. Nieuwenhuis. 1987. B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens. Eur.-J. Immunol, 17: 921.PubMedCrossRefGoogle Scholar
  3. 3.
    Hunt, S.V. and A.F. Williams. 1974. The origin of cell surface immunoglobulin of marrow-derived and thymus-derived lymphocytes in the rat. J. Exp. Ked, 139: 479CrossRefGoogle Scholar
  4. 4.
    Ford, V.L. 1977. Preparation and labelling of lymphocytes. In Handbook of Experimental Immunology. D.M. Weir, editor. Blackwell Scientific Publications, Oxford, England, 3rd edition. Chapter 23.Google Scholar
  5. 5.
    Parks, D.E., L.L. Lanier and L.A. Herzenberg. 1986. Flow cytometry and fluorescence activated cell sorting (FACS), In Handbook of Experimental Immunology, D. M, Weir, editor. Blackwell Scientific Publications, Oxford, England. 4th edition. Chapter 29.Google Scholar
  6. 6.
    Finkleman, F.D., I. Scher, J.J. Mond, J.T. Kung, and E.S. Metcalf. 1982. Polyclonal activation of the murine immune system by an antibody to IgD I. Increase in cell size and DIA synthesis. J. Immunol, 129: 629.Google Scholar
  7. 7.
    Monroe, J.G., V.L. Havran, and J.C. Cambier. 1983. B lymphocyte activation: entry into cell cycle is accompanied by decreased expression of IgD but not IgM. Eur. J. Immunol, 13: 208.PubMedCrossRefGoogle Scholar
  8. 8.
    Seijen, H.G., J.C.A.M. Bun, A.S. Vubbena, and K.G. L. Löhlefink, The germinal centre precursor cell is surface μ and σ positive, this symposium.Google Scholar
  9. 9.
    deKruyff, H.H., S. R. Onikul, and G.J. Thorbecke. 1976. Migratory patterns of B lymphocytes, V, Surface Ig and migration properties of density gradient-separated bursa cells. Eur. J. Immunol, 6: 462.PubMedCrossRefGoogle Scholar
  10. 10.
    Coico, R.F. and G.J. Thorbecke. 1985. Hole of GC in the generation of B cell memory. Folia Microbiol. 30: 196.CrossRefGoogle Scholar
  11. 11.
    Gray, D. Memory B cells but not virgin B cells are activated in germinal centres, this symposium.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • R. H. Vonderheide
    • 1
  • S. V. Hunt
    • 1
  1. 1.Sir William Dunn School of PathologyUniversity of OxfordEngland

Personalised recommendations