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Isolation of Follicular Dendritic Cells from Human Tonsils and Adenoids. in Vitro Culture

  • N. Cormann
  • E. Heinen
  • C. Kinet-Denoel
  • R. Tsunoda
  • L. J. Simar
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)

Abstract

Germinal centers of lymphoid follicles are made of lymphoid cells and of accessory cells. Among these are the follicular dendritic cells or FDC’s. They surround lymphoid cells with fine membrane extensions and we have shown that those closed relations are preserved after isolation of the cells from lymphoid tissues (1,2,3,4). To analyze the interactions that may take place between FDC and lymphocytes in such associations, we wanted keep them alive in vitro. For this purpose, we tried to maintain in culture entire human follicles obtained after dissection under biomicroscope and, secondly, to cultivate isolated human FDC. On an other hand we hoped to maintain pure FDC in culture, that means FDC populations without any accompanying lymphocyte. For this purpose we used a culture technic described by Jordan et al. (5). They obtained a rapid degeneration of mice thymic lymphocytes when the cultures were performed at 24°C.

Keywords

Lymphoid Cell Germinal Center Nurse Cell Lymphoid Follicle Organotypic Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Lilet-Leclercq C., Radoux D., Heinen E., Kinet-Denoël C., Defraigne J.O., Houben-Defresne M.P., Simar L.J. J. Immunol. Meth. 66: 235 (1984).CrossRefGoogle Scholar
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • N. Cormann
    • 1
  • E. Heinen
    • 1
  • C. Kinet-Denoel
    • 1
  • R. Tsunoda
    • 1
  • L. J. Simar
    • 1
  1. 1.Institute of human histologyUniversity of LiègeLiègeBelgium

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