Heterogeneity in the Expression of B-Cell Antigen in Non-Hodgkin’s Lymphoma
The value of monoclonal antibodies (MoAbs) for the identification of neoplastic B-cells is now undisputed. Studies of normal B-cell populations with large series of MoAbs have demonstrated tha distinct markers are acquired and lost during ontogeny.I,Z Workshops on human leucocyte differentiating antigens have described a universal system for MoAb classification, MoAbs falling into the same cluster of differentiation, or CD group, bind to the same B-cell monoclonal antigen but may bind to different epitopes on that molecule. 3 The majority of pan-B reagents identify the bulk of B-cell malignancies, although the efficiency of detection varies in different histolgical types of tumour and with different CD specificities.4,5,6 In this presentation we have reviewed the performance of a range of B-cell antibodies in our routine diagnostic profiles. We report on variation in the staining obtained with different reagents and also present new data on the gfficacy of two locally produced antibodies, WR147 and WR178 in the diagnosis of B-non-Hodgkin’s lymphoma (B-NHL).
KeywordsLymphoplasmacytic Lymphoma Leucocyte Typing Lineage Antigen Follicle Centre Cell CD22 MoAb
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- 1.L M Nadler, K C Anderson, M Bates, E Park, B L Slaughenhoupt and S F Schlossman. In: ‘Leucocyte Typing’. Springer-Verlag: Berlin: 354 (1984).Google Scholar
- 2.L M Nadler, P Stashenko, R Hardy, A van Agthoven, C Terhorst and S F Schlossman. J Immunol 126: 1941 (1983).Google Scholar
- 7.K Moore, S A Cooper, A M Nesbitt and D B Jones. Submitted for publication.Google Scholar
- 14.R Schwartz. Personal communication.Google Scholar
- 18.A Pezzutto, A Dorken, G Feller, R Moldenhauer, R Schwartz. In: Leucocyte Typing II, Vol 2. Springer-Verlag: Berlin: 47 (1986).Google Scholar