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IgG Subclass Distribution in Juvenile Human Tonsil: IgG3 and IgG4 Results of Specific Antibody Production Using Synthetic Peptides

  • W. J. A. Boersma
  • C. Deen
  • N. D. Zegers
  • J. Radl
  • J. J. Haaijman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)

Summary

A decapeptide with a sequence corresponding to a part of the hinge region of human IgG3 was used to prepare a mouse monoclonal antibody (Mab 330-2.2). The Mab recognized IgG3 in ELISA only when the IgG3 was denatured by mild heat. Mab specificity in immunohistochemistry was calibrated with a number of reference Mabs obtained through a WHO/IUIS collaborative study. Finally, Mab 330-2.2 was used in conjunction with a set of other isotype specific Mabs to study the (sub) class distribution of cytoplasmic Ig containing cells in palatine tonsil. IgG3 within the IgG class was strongly over-represented in our tonsil material if compared to the representation of IgG3 in serum.

Keywords

Hinge Region Palatine Tonsil Immunology Today Native IgG3 Repeated Amino Acid Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • W. J. A. Boersma
    • 1
  • C. Deen
    • 1
  • N. D. Zegers
    • 1
  • J. Radl
    • 2
  • J. J. Haaijman
    • 1
  1. 1.Medical Biological Lab. TNORijswijkThe Netherlands
  2. 2.Institute for Experimental Gerontology TNORijswijkThe Netherlands

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