Characterization of Cell Populations, Reappearing in the Mouse Spleen After Elimination by Liposome Encapsulated Dichloromethylene Diphosphonate: Reappearance of Marginal Zone Lymphocytes is Independent of Marginal Zone Macrophages

  • Nico van Rooijen
  • Nel Kors
  • Georg Kraal
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


Macrophages in the spleen can be eliminated by treatment with liposome encapsulated dichloromethylene diphosphonate (DMDP; reviewed in ref. 1. These macrophages disappeared during the first day after intravenous injection with liposome-encapsulated DMDP, remained absent for one week and reappeared gradually in the next weeks, as studied with the aid of enzyme histochemical techniques (2). Macrophages in different compartments of the mouse spleen can now be characterized by various monoclonal antibodies (3–6).


Marginal Zone Mouse Spleen White Pulp Single Intravenous Injection Multilamellar Liposome 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Nico van Rooijen
    • 1
  • Nel Kors
    • 1
  • Georg Kraal
    • 1
  1. 1.Immunocytochemistry Unit, Department of Histology, Medical FacultyFree UniversityAmsterdamThe Netherlands

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