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Germinal Center B Cells Present Antigen Obtained in vivo to T Cells in vitro and Stimulate Mixed Lymphocyte Reactions

  • Marie H. Kosco
  • Alan P. Monfalcone
  • Andras K. Szakal
  • John G. Tew
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)

Abstract

Shortly after secondary immunization, germinal center (GC) B cells have been observed in ultrastructural studies to obtain antigen from follicular dendritic cells (FDCs) in the form of immune complexes (1). This antigen appears to be degraded by GC B cells and may be processed for presentation to T cells. The present study was undertaken to determine if GC B cells can process and present antigen obtained from FDCs in vivo to appropriate T cells in vitro. In addition, the stimulatory capacity of GC B cells on T cells was evaluated in primary mixed lymphocyte reactions (MLR). The findings presented here demonstrate that antigen obtained in vivo by GC B cells can be processed and presented to T cells. The ability of GC B cells to interact with T cells is indicated by the fact that they served as potent stimulators of allogeneic T cells. In contrast, the small high density (HD) B cells are weak to negative stimulators under the same conditions. In addition, the GC B cells were even able to stimulate detectable syngeneic MLRs. These data suggest that in vivo, GC B cells are capable of interacting with T cells, in turn inducing the T cell help needed for the germinal center reaction, generating B memory cells, and influencing the high titers of antibody associated with the secondary antibody response.

Keywords

Germinal Center Potent Stimulator Mixed Lymphocyte Reaction Follicular Dendritic Cell Stimulatory Capacity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Marie H. Kosco
    • 1
    • 2
  • Alan P. Monfalcone
    • 1
    • 2
  • Andras K. Szakal
    • 1
    • 2
  • John G. Tew
    • 1
    • 2
  1. 1.Department of Microbiology and ImmunologyMedical College of Virginia, VCURichmondUSA
  2. 2.Department of Anatomy and ImmunobiologyMedical College of Virginia, VCURichmondUSA

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