Morphological Studies on Mitogen and Antigen Presentation by Human and Rabbit Cells
Mitogen presentation was studied using human peripheral blood cells from normal subjects. The technique involved defibrination of the blood, separation of the mononuclear cells on Ficoll, followed by removal of adherent macrophages. The non-adherent cells were separated on a metrizamide gradient into a low density fraction enriched for dendritic cells (LDC) and a high density fraction containing lymphocytes. (1) The lymphocytes were further separated into T and non-T fractions by rosetting with neuraminidase-treated sheep red blood cells (SRBC). The LDC fraction was pulsed with pokeweed mitogen (PWM) 100 ng/ml and mixed with autologous lymphocytes in the ratio of one LDC to ten lymphocytes. The lymphocyte fraction contained T and non-T cells in the ratio of two to one.
KeywordsPeripheral Mononuclear Cell High Power View High Density Fraction Keyhole Limpet Haemocyanin Adherent Macrophage
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