The Effects of Bacterial Lipopolysaccharide (LPS) on High Endothelial Venules, T Lymphocytes and Interdigitating Cells in Mouse Lymph Nodes

  • Agaath J. T. Twisk
  • Paul H. P. Groeneveld
  • Georg Krall
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


Lymphocytes can recirculate continuously throughout the body from blood to lymph and from lymph to blood. The lymphocyte extravasation from blood into lymphoid tissues is mediated by a specialized endothelium, morphologically characterized by high, cuboidal cells, found in venules, located in lymph nodes and mucosal lymphoid organs (1, 2). Recent studies suggest that lymphocyte migration through the wall of these so-called high endothelial venules (HEV) is accomplished by a ligand receptor system present on the endothelium of HEV and on the surface of lymphocytes, which differs between lymph nodes and Peyer’s patches. This organ-specific HEV-lymphocyte interaction selectively controls the lymphocyte migration through the body (3–7). Little is known about the mechanisms that regulate the activity of the HEV and the specificity of interaction with lymphocytes in various lymphoid organs. In the lymph node HEV are located in the T cell dependent areas. These areas are characterized by the presence of non-lymphoid cells, the interdigitating cell (IDC), which may play an essential role in maintaining the integrity of the T cell area. The aim of this study was to determine whether by disturbing the integrity of the T cell area the function of the HEV could be influenced. This was studied in mice by injecting bacterial lipopolysaccharide (LPS) subcutaneously in the hind footpad. LPS was chosen because of its effects on IDC (8) and T cells (9) in the mouse spleen. Using immunohistochemistry the influence of LPS on the T cell dependent areas was examined and using short-term in vivo homing experiments special attention was paid to the HEV specificity.


Lymphoid Organ Cell Area Popliteal Lymph Node High Endothelial Venule Lymphocyte Migration 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Agaath J. T. Twisk
    • 1
  • Paul H. P. Groeneveld
    • 1
  • Georg Krall
    • 1
  1. 1.Department of Histology, Medical FacultyFree UniversityAmsterdamThe Netherlands

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