Expression and Function of MHC Class II Antigens on Macrophages and Dendritic Cells

  • E. W. A. Kamperdijk
  • M. A. M. Verdaasdonk
  • R. H. J. Beelen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


Macrophages bearing MHC class II antigens (Ia antigens) play an important role in presenting antigen to specifically primed T lymphocytes(1). Since populations of macrophages are heterogeneous in the expression of detectable Ia antigen (2,3), the presence of the determinant marks the macrophage subpopulation responsible for this important immunoregulatory function. It has been shown that also dendritic cells (4) play a crucial role in antigen presentation to immunocompetent T lymphocytes. In this study we have investigated the expression and function of Ia antigen on different populations of macrophages and dendritic cells from inbred ACI/Mal rats, which are genetically defined high responders for the copolymer GT (glutamine tyrosine, 5). The expression of Ia antigen was detected by EA rosetting on both vital and paraformaldehyde fixed cells and by immunocytochemistry (immunoperoxidase).


Dendritic Cell Black Reaction Derive Macrophage Bone Marrow Chromic Chloride Resident Peritoneal Macrophage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    E.R. Unanue. Adv. Immunol. 31: 1 (1981).Google Scholar
  2. 2.
    C. Cowing, B.D. Schwartz, and H.B. Dickler. J. Immunol. 120: 378 (1978).PubMedGoogle Scholar
  3. 3.
    H.R. Schwartz, H.B. Dickler, D.H. Sachs, and B.D. Schwartz. Scand. J. Immunol. 5: 731 (1976).PubMedCrossRefGoogle Scholar
  4. 4.
    R.M. Steinman, and Z.A. Cohn. J. Exp. Med. 139: 380 (1974).PubMedCrossRefGoogle Scholar
  5. 5.
    D. Amerding, D.H. Katz, and B. Benacerraf. Immunogenetics 1: 329 (1974).CrossRefGoogle Scholar
  6. 6.
    E.C.M. Hoefsmit, and R.H.J. Beelen, in Mononuclear phagocytes. Characteristics, physiology and function, p. 19, R. van Fürth, ed., Martinus Nijhof, Dordrecht (1985).Google Scholar
  7. 7.
    W.S. Walker, R.B. Hester, and R.H.J. Beelen. Cell. Immunol. 79: 125 (1983).PubMedCrossRefGoogle Scholar
  8. 8.
    E.W.A. Kamperdijk, M.L. Kapsenberg, M. van den Berg, and E.C.M. Hoefsmit. Cell Tissue Res. 242: 469 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    C.R. Parish, and J.A. Hayward. Proc. R. Soc. Lond. Ser. B 197: 47 (1974).CrossRefGoogle Scholar
  10. 10.
    D.I. Beller, and E.R. Unanue. J. Immunol. 126: 263 (1981).PubMedGoogle Scholar
  11. 11.
    E.C.M. Hoefsmit, I.L. Schadee-Eestermans, and R.H.J. Beelen. J. Histo-chem. Cytochem. 34: 633 (1986).CrossRefGoogle Scholar
  12. 12.
    E.G. Calamai, D.I. Beller, and E.R. Unanue. J. Immunol. 128: 1692 (1982).PubMedGoogle Scholar
  13. 13.
    W.S. Walker, R.H.J. Beelen, P.J. Buckley, S.L. Melvin, and S. Yen. J. Immunol. Methods 67: 89 (1984).PubMedCrossRefGoogle Scholar
  14. 14.
    E.W.A. Kamperdijk, M. van den Berg, M.L. Kapsenberg, and E.C.M. Hoefsmit. Cell Biol. Int. Rep. 8: 180 (1984).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • E. W. A. Kamperdijk
    • 1
  • M. A. M. Verdaasdonk
    • 1
  • R. H. J. Beelen
    • 1
  1. 1.Department of Cell Biology, Medical FacultyFree UniversityAmsterdamThe Netherlands

Personalised recommendations