J Chain is Expressed by More IgA2-than IgA1-Producing Cells in Colonic Mucosa, but it is Reduced in Both Subclasses in Inflammatory Bowel Disease

  • K. Kett
  • P. Brandtzaeg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


We have recently observed a shift from the normal predominance of IgA2- (1) to IgA1- producing cells in colonic mucosa from patients with ulcerative colitis (UC) and Crohn’s colitis (CC) (2). This disease-associated alteration may be of great biological importance in terms of secretory immunity. Only J-chain-containing polymeric immunoglobulins(pIg), such as dimeric IgA (pIgA) and pentameric IgM will bind to secretory component (SC) which functions as a pig transport receptor on secretory epithelial cells (3). In this study the capacity of mucosal IgA1- and IgA2-producing cells for plgA formation was examined by paired immunofluorescence staining for subclass and J chain, both in normal and inflamed colonic tissue specimens.


Ulcerative Colitis Colonic Mucosa Secretory Component Secretory Epithelial Cell Heavy Chain Constant Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    K. Kett, P. Brandtzaeg, J. Radl, J. J. Haaijman, Different subclass distribution of IgA-producing cells in human lymphoid organs and various secretory tissues, J. Immunol. 136: 3651 (1986).Google Scholar
  2. 2.
    K. Kett, P. Brandtzaeg, Local IgA subclass alterations in ulcerative colitis and Crohn’s disease of the colon, Gut 28: 1013 (1987).PubMedCrossRefGoogle Scholar
  3. 3.
    P. Brandtzaeg, H. Prydz, Direct evidence for an integrated function of J chain and secretory component in epithelial transport of immunoglobulins, Nature 311: 71 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    P. Brandtzaeg, Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique, Immunology 26: 1101 (1974).PubMedGoogle Scholar
  5. 5.
    P. Brandtzaeg, Immunohistochemical characterization of intracellular J-chain and binding site for secretory component (SC) in human immunoglobulin (Ig)-producing cells, Mo1ec. Immuno1. 20: 941 (1983).CrossRefGoogle Scholar
  6. 6.
    P. Brandtzaeg, K. Kett, T. O. Rognum, et al, Distribution of mucosal IgA and IgG subclass-producing immunocytes and alterations in various disorders, Monogr. Allergy 20: 179 (1986).PubMedGoogle Scholar
  7. 7.
    S. S. Crago, W. H. Kutteh, I. Moro, et al, Distribution of IgAl-, IgA2-, and J chain-containing cells in human tissues, J. Immunol. 132: 16 (1984).PubMedGoogle Scholar
  8. 8.
    P. Brandtzaeg, F. R. Korsrud, Significance of different J chain profiles in human tissues: generation of IgA and IgM with binding site for secretory component is related to the J chain expressing capacity of the total local immunocyte population, including IgG and IgD producing cells, and depends on the clinical state of the tissue, Clin. exp. Immunol. 58: 709 (1984).PubMedGoogle Scholar
  9. 9.
    K. Kett, T. O. Rognum, P. Brandtzaeg, Mucosal subclass distribution of Immunoglobulin G-producing cells is different in ulcerative colitis and Crohn’s disease of the colon, Gastroenterology (in press).Google Scholar
  10. 10.
    J. G. Flanagan, T. H. Rabbits, Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing γ, ε, and a genes, Nature 300: 709 (1982).PubMedCrossRefGoogle Scholar
  11. 11.
    L. Hammarström, H. Mellstedt, M. A. A. Persson, C. I. E. Smith, A. Ahre, IgA subclass distribution in paraproteinemias: suggestion of an IgG-IgA subclass switch pattern, Acta Pathol. Microbiol. Immunol. Scand. (C) 92: 207 (1984).Google Scholar
  12. 12.
    M. Takahashi, T. Tsukada, M. Kojima, et al, Immunoglobulin class switch from IgG to IgA in a patient with smoldering multiple myeloma, Blood 67: 1710 (1986).PubMedGoogle Scholar
  13. 13.
    P. Brandtzaeg, Research in gastrointestinal immunology — state of the art, Scand. J. Gastroenterol. 20 (Suppl. 114): 137 (1985).CrossRefGoogle Scholar
  14. 14.
    P. Brandtzaeg, K. Vaines, H. Scott, T. O. Rognum, K. Bjerke, K. Baklien, The human gastrointestinal secretory immune system in health and disease, Scand. J. Gastroenterol. 20 (KSuppl. 114): 17 (1985).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • K. Kett
    • 1
  • P. Brandtzaeg
    • 1
  1. 1.Laboratory for Immunohistochemistry and Immunopathology (LIIPAT)Institute of Pathology, University of Oslo RikshospitaletOslo 1Norway

Personalised recommendations