Differential Expression of Epithelial MHC Class II Determinants (HLA-DR, -DP, and -DQ) and Increased Class I Expression in Inflamed Salivary Glands

  • Per S. Thrane
  • Per Brandtzaeg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 237)


Sjögren’s syndrome (SS) affects salivary and lacrimal glands, causing xerostomia and keratoconjunivitis. An increased number of infiltrating leukocytes are present and the glandular tissue is gradually destroyed. Increased epithelial HLA-DR expression in salivary glands from SS patients has been reported (1,2,3), but the other MHC class II subregion products(HLA-DP and -DQ) and MHC class I determinants have not been studied.


Salivary Gland Pleomorphic Adenoma Salivary Gland Tissue Salivary Gland Epithelial Cell Epithelial Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    G. Lindahl, E. Hedfors, L. Klareskog and U. Forsum, Epithelial HLA-DR expression and T lymphocyte subsets in salivary glands in Sjögren’s syndrome, Clin. Exp. Immunol. 61: 475 (1985).PubMedGoogle Scholar
  2. 2.
    R. I. Fox, T. Bumol, R. Fantozzi, R. Bone and R. Schreiber, Expression of histocompatibility antigen HLA-DR by salivary gland epithelial cells in Sjogren’s syndrome, Arthr. Rheum. 29: 1105 (1986).CrossRefGoogle Scholar
  3. 3.
    H. M. Moutsopoulos, J. J. Hooks, C. C. Chan, Y. A. Dalavanga, F. N. Skopouli and B. Detrick, HLA-DR expression by labial minor salivary gland tissues in Sjogren’s syndrome. Ann. Rheum. Pis. 45: 677 (1986).CrossRefGoogle Scholar
  4. 4.
    G. F. Bottazzo, R. Pujol-Borrell and T. Hanafusa, Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity, Lancet ii: 1115 (1983).CrossRefGoogle Scholar
  5. 5.
    I. Todd, M. Londel, R. Pujol-Borrell, R. Mirakian, M. Feldmann and G. F. Bottazzo, HLA-D/DR expression on epithelial cells: the finger on the trigger?, Ann. N.Y. Acad. Sci. 475: 241 (1986).PubMedCrossRefGoogle Scholar
  6. 6.
    J. B. Harley, M. Reichlin, F. C. Arnett, E. L. Alexander, W. B. Bias and T. T. Provost, Gene interaction at HLA-DQ enhances autoantibody production in primary Sjogren’s syndrome, Science 232: 1145 (1986).PubMedCrossRefGoogle Scholar
  7. 7.
    R. I. Fox, A. N. Theofilopoulos and A. Altman, Production of inter-leukin 2 (IL 2) by salivary gland lymphocytes in Sjogren’s syndrome. Detection of reactive cells by using antibody directed to synthetic peptides of IL 2, J. Immunol. 135: 3109 (1985).PubMedGoogle Scholar
  8. 8.
    M. Segerberg-Konttinen, V. Bergroth, P. Jungeil, M. Malmström, D. Norström, J. Sane, I. Immonen and Y. T. Konttinen, Activated lymphocytes and lymphoblasts in the minor salivary glands of patients with Sjogren’s syndrome, Scand. J. Immuno1. 24: 79 (1986).Google Scholar
  9. 9.
    D. A. Isenberg, D. Rowe, A. Tookman, A. Hopp, Griffiths, E. Paice, J. Stewart and P. C. L. Beverley, An immunohistological study of secondary Sjogren’s syndrome, Ann. Rheum. Dis. 43: 470 (1984).PubMedCrossRefGoogle Scholar
  10. 10.
    R. Pujol-Borell, I. Todd, M. Doshi, G.F. Bottazzo, R. Sutton, D. Gray, G.R. Adolf and M. Feldmann, HLA class II induction in human islet cells by interferon-plus tumour necrosis factor or lymphotoxin, Nature 326: 304 (1987).CrossRefGoogle Scholar
  11. 11.
    K. Pfizenmaier, P. Scheurich, C. Scluter and M. Krönke, Tumor necrosis factor enhances HLA-A,B,C and HLA-DR gene expression in human tumor cells, J. Immunol. 138: 975 (1987).PubMedGoogle Scholar
  12. 12.
    T. E. Daniels, Labial salivary gland biopsy in Sjogren’s syndrome. Assessment as a diagnostic criterion in 362 suspected cases, Arthr. Rheum. 27: 147 (1984).CrossRefGoogle Scholar
  13. 13.
    P. S. Thrane, L. M. Sollid, P. Brandtzaeg and H. R. Haanes, Increased expression of secretory component (SC) and HLA-DR in salivary gland epithelium from patients with Sjogren’s syndrome, submitted (1987).Google Scholar
  14. 14.
    P. Brandtzaeg, Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with cold ethanol-fixation technique, Immunology 26: 1101 (1974).PubMedGoogle Scholar
  15. 15.
    P. Brandtzaeg and T. O. Rognum, Evaluation of tissue preparation methods and paired immunofluorescence staining for immunocyto-chemistry of lymphomas, Histochem. J. 15: 655 (1983).PubMedCrossRefGoogle Scholar
  16. 16.
    D. Y. Mason, Techniques in Immunohistochemistry, vol. 3, 25–43 (1985).Google Scholar
  17. 17.
    P. Brandtzaeg, I. Dale and M. K. Fagerhol, Distribution of a formalin-resistant myelimonocytic antigen (L1) in human tissues. I. Comparison with other leukocyte markers by paired immunofluorescence and immuinoenzyme staining, Am J. Clin. Pathol. 87: 681 (1987).PubMedGoogle Scholar
  18. 18.
    L. A. Lampson and R. Levy, Two populations of la-like molecules on a human B cell line, J. Immunol. 125: 293 (1980).PubMedGoogle Scholar
  19. 19.
    Y. X. Chen, R. L. Evans, M. S. Pollack, L. L. Lanier, J. H. Phillips, C. Rousso, N. L. Warner and F. M. Brodsky, Characterization and expression of the HLA-DC antigens defined by anti-Leu 10, Hum. Immunol. 10: 221 (1984).PubMedCrossRefGoogle Scholar
  20. 20.
    A. J. Watson, R. DeMars, I.S. Trowbridge and F.H. Bach, Detection of a novel class II HLA antigen, Nature 304: 358 (1983).PubMedCrossRefGoogle Scholar
  21. 21.
    C. J. Barnstable, W. F. Bodmer, G. Brown, G. Galfre, C. Milstein, A. F. Williams and A. Ziegler, Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens — new tools for genetic analysis, Cell 14: 9 (1978).PubMedCrossRefGoogle Scholar
  22. 22.
    J. A. Ledbetter, R. E. Evans, M. Lipinski, C. Cunningham Rundles, R. A. Good and L. A. Hertzenberg, Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and T cytotoxic/supressor subpopulations in mouse and man, J. Exp. Med. 153: 310 (1981).PubMedCrossRefGoogle Scholar
  23. 23.
    H. Huitfeldt and P. Brandtzaeg, Various keratin antibodies produce immunohistochemical staining of human myoepithelium and myometrium, Histochemistry 83: 381 (1985).PubMedCrossRefGoogle Scholar
  24. 24.
    Y. A. Dalavanga, B. Detrick, J. J. Hooks, A. A. Droosos and H. M. Moutsopoulos, Effect of cyclosporin A (CyA) on the immunopatho-logical lesion of the labial minor salivary glands from patients with Sjogren’s syndrome, Ann. Rheum. Dis. 47: 89 (1987).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Per S. Thrane
    • 1
    • 2
  • Per Brandtzaeg
    • 1
  1. 1.Laboratory for Immunohistochemistry and Immunopathology (LIIPAT)Institute of Pathology, Medical Faculty, The National Hospital, RikshospitaletOslo 1Norway
  2. 2.Department of Oral Surgery and Oral Medicine, Dental FacultyUniversity of OsloOslo 1Norway

Personalised recommendations