Comparison of Growth Effects of rhIGF-l in Diabetic and Hypox Rats with those of Insulin and Growth Hormone

  • E. R. Froesch
  • H. P. Guler
  • M. Ernst
  • C. Schmid
  • E. Scheiwiller
  • J. Zapf
Part of the Serono Symposia, USA book series (SERONOSYMP)


Insulin-like growth factor I (IGF-I) stimulates replication of cultured cells in vitro (1–3). In contrast to other growth factors, IGF-I does not lead to dedifferentiation of differentiated cells in culture, but rather maintains or even enhances the degree of differentiation of cultured cells (2,4,5). In view of (1) these in vitro findings, (2) the similarity of action between insulin and IGF-I on differentiation (2–5), and (3) several reports on growth stimulation by IGF-I in hypox and other animals (6–8), we decided to investigate the action of recombinant human IGF-I (rhIGF-I) on diabetic and hypophysectomized rats. In addition, acute effects of IGF yere studied in man (9,10).


Growth Hormone Free Fatty Acid Level Longitudinal Bone Growth Osteoblastlike Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Morell B, Froesch ER. Fibroblasts as an experimental tool in metabolic and hormone studies. II. Effects of insulin and non- suppressible insulin-like activity (NSILA-s) on fibroblasts in culture. Eur J Clin Invest 1973; 3: 119.Google Scholar
  2. 2.
    Schmid C, Steiner T, Froesch ER. Preferential enhancement of myoblast differentiation by insulin-like growth factors (IGF-I and IGF-II) in primary cultures of chicken embryonic cells. FEBS Lett 1983; 161: 117.PubMedCrossRefGoogle Scholar
  3. 3.
    Schmid C, Steiner T, Froesch ER. Insulin-like growth factor I supports differentiation of cultured osteoblast-like cells. FEBS Lett 1984; 173: 48.PubMedCrossRefGoogle Scholar
  4. 4.
    Ernst M, Froesch ER. Osteoblastlike cells in a serum-free methylcellulose medium form colonies: effects of insulin and insulinlike growth factor I. Calcif Tissue Int 1987; 40: 27.PubMedCrossRefGoogle Scholar
  5. 5.
    Froesch ER, Schmid C, Zangger I, Schoenle E, Eigenmann E, Zapf J. Effects of IGF/somatomedins on growth and differentiation of muscle and bone. J Anim Sci 1986; 63 (suppl 2): 57.Google Scholar
  6. 6.
    Schonle E, Zapf J, Humbel RE, Froesch ER. Insulin-like growth factor I stimulates growth in hypophysectomized rats. Nature 1982; 296: 252.CrossRefGoogle Scholar
  7. 7.
    Scheiwiller E, Guler HP, Merryweather J, et al. Growth restoration of insulin-deficient diabetic rats by recombinant human insulin-like growth factor I. Nature 1986; 323: 169.PubMedCrossRefGoogle Scholar
  8. 8.
    Van Buul-Offers S, Ueda I, Van den Brande JL. Biosynthetic somatomedin C (SM-C/IGF-I) increases the length and weight of snell dwarf mice. Pediatr Res 1986; 20: 825.PubMedCrossRefGoogle Scholar
  9. 9.
    Guler HP, Zenobi P, Zapf J, et al. IGF-I and II and recombinant human (rh) IGF-I are hypoglycemic in the rat, mini-pig and men [Abstract]. Anaheim, CA: The Endocrine Society, 1986.Google Scholar
  10. 10.
    Guler HP, Zapf J, Froesch ER. Acute metabolic effects of recombinant human insulin-like growth factor I (rhIGF-I) in healthy adult men. N Engl J Med (in press) 1987.Google Scholar
  11. 11.
    Zapf J, Hauri C, Waldvogel M, Froesch ER. Acute metabolic effects and half-lives of intravenously administered insulinlike growth factors I and II in normal and hypophysectomized rats. J Clin Invest 1986; 77: 1768.PubMedCrossRefGoogle Scholar
  12. 12.
    Oelz O, Jakob A, Froesch ER. Non-suppressible insulin-like activity (NSILA) of human serum. V. Hypoglycaemia and preferential metabolic stimulation of muscle by NSILA-S. Eur J Clin Invest 1970; 1: 48.PubMedCrossRefGoogle Scholar
  13. 13.
    Froesch ER. Nonsuppressible insulin-like activity of human serum. II. Biological properties of plasma extracts with nonsuppressible insulin-like activity. Biochim Biophys Acta 1966; 121: 360.PubMedCrossRefGoogle Scholar
  14. 14.
    Froesch ER, Zapf J. Insulin-like growth factors and insulin: comparative aspects. Diabetologia 1985; 28: 485.PubMedCrossRefGoogle Scholar
  15. 15.
    Froesch ER, Zapf J. Insulin, IGF-I and growth in diabetic rats [Reply]. Nature 1987; 326: 549.CrossRefGoogle Scholar
  16. 16.
    Guler HP, Zapf J, Froesch ER. infusion of recombinant human insulin-like growth factor I (rhIGF-I) stimulates growth of hypophysectomized rats continuously during 18 days [Abstract]. Copenhagen: First European Congress of Endocrinology, 1987.Google Scholar
  17. 17.
    Guler HP, Zapf J, Scheiwiller E, Froesch ER. Recombinant human insulin-like growth factor I stimulates growth and has specific effects on organ size in hypophysectomized rats. (Submitted.)Google Scholar
  18. 18.
    Kurtz A, Jelkmann W, Bauer C. A new candidate for the regulation of erythropoiesis. FEBS Lett 1982; 149: 105.PubMedCrossRefGoogle Scholar
  19. 19.
    Bernier M, Chatelain P, Mather JP, Saez JM. Regulation of gonadotropin receptors, gonadotropin responsiveness, and cell multiplication by somatomedin-C and insulin in cultured pig Leydig cells. J Cell Physiol 1986; 129: 257.PubMedCrossRefGoogle Scholar
  20. 20.
    Zapf J, Walter H, Froesch ER. Radio immunological determination of insulin-like growth factors I and II in normal subjects and in patients with growth disorders and extrapancreatic tumor hypoglycemia. J Clin Invest 1981; 68: 1321.PubMedCrossRefGoogle Scholar
  21. 21.
    Poggi C, le Marchand-Brustel Y, Zapf J, Froesch ER, Freychet P. Effects and binding of insulin-like growth factor I in the isolated soleus muscle of lean and obese mice: comparison with insulin. Endocrinology 1979; 105: 723.PubMedCrossRefGoogle Scholar
  22. 22.
    Meuli C, Froesch ER. Insulin and nonsuppressible insulin-like activity (NSILA-S) stimulate the same glucose transport system via two separate receptors in rat heart. Biochem Biophys Res Commun 1977; 75: 689.PubMedCrossRefGoogle Scholar
  23. 23.
    Vetter U, Zapf J, Heit W, et al. Human fetal and adult chondrocytes. Effect of insulinlike growth factors I and II, insulin, and growth hormone on clonal growth. J Clin Invest 1986; 77: 1903.PubMedCrossRefGoogle Scholar
  24. 24.
    Widmer U, Schmid C, Zapf J, Froesch ER. Effects of insulin-like growth factors on chick embryo hepatocytes. Acta Endocrinol (Copenh) 1985; 108: 237.Google Scholar
  25. 25.
    Salmon WD Jr, Daughaday WH. A hormonally controlled serum factor which stimulates sulfate incorporation into cartilage. J Lab Clin Med 1957; 49: 825.PubMedGoogle Scholar
  26. 26.
    Veldhuis JD, Furlanetto RW. Trophic actions of human somatomedin C/insulin-like growth factor I in ovarian cells: in vitro studies with swine granulosa cells. Endocrinology 1985; 116: 1235.PubMedCrossRefGoogle Scholar
  27. 27.
    Isaksson OGP, Eden S, Jansson J-O. Mode of action of pituitary target cells. Annu Rev Physiol 1985; 47: 483.PubMedCrossRefGoogle Scholar
  28. 28.
    Nixon BT, Green H. Growth hormone promotes the differentiation of myoblasts and preadipocytes generated by azacytidine treatment of 10 T\cells. Proc Natl Acad Sci USA 1984; 81: 3429.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • E. R. Froesch
    • 1
  • H. P. Guler
    • 1
  • M. Ernst
    • 1
  • C. Schmid
    • 1
  • E. Scheiwiller
    • 1
  • J. Zapf
    • 1
  1. 1.Metabolic Unit, Department of MedicineUniversity of ZurichZurichSwitzerland

Personalised recommendations