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Combined Use of Lectin Histochemistry and Immunocytochemistry for the Study of Neurosecretion

  • E. M. Rodriguez
  • B. Peruzzo
  • L. Alfaro
  • H. Herrera

Abstract

In 1971, on the occasion of a Symposium organized by Heller and Lederis, and also held in Bristol, I presented some histochemical evidence indicating that in the neural lobe of amphibians and reptilian only one of the two types of neurosecretory fibers contained carbohydrates. More substantial evidence was provided by Tasso (1974) who, working with rats at the ultrastructural level, demonstrated that glycoproteins were circumscribed to neurosecretory granules located in one of the two types of neurosecretory fibers. Later, Tasso et al., (1977), by studying the hypothalamo-neurohypophysial system of Brattleboro rats, showed that the neurosecretory granules containing glycoproteins were missing from homozygous rats and postulated that glycoproteins and arginine-vasopressin (AVP) coexisted within the same granule. Apparently, no further histochemical studies have been performed.

Keywords

Sialic Acid Rough Endoplasmic Reticulum Sugar Residue Neurosecretory Granule Lectin Histochemistry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Gainer, H., 1981, The biology of neurosecretory neurons, in: “Neurosecretion and Brain Peptides”, J.B. Martin, S. Reichlin, and K.L. Bick, eds., Raven Press, New York.Google Scholar
  2. Goldstein, I.J., and Hayes, C.E., 1978, The lectins: Carbohydrate binding proteins of plants and animals. Adv. Carbohvdr. Chem. Biochem., 35: 127.CrossRefGoogle Scholar
  3. Haddad, A., Pelletier, G., Marchi, F., and Brasileiro, L., 1976, Radioautographic study of glycoprotein biosynthesis migration and renewal in the hypothalamusneurohypophysis system, Proc. 5th Colloa. Brazilian Soc. Elect. Microsc., p.62.Google Scholar
  4. Holwerda, D.A., 1972, A glycopeptide from the posterior lobe of pig pituitaries, Eur. J. Biochem., 28: 340.CrossRefGoogle Scholar
  5. Ivell, R., Schmale, H., and Richter, D., 1981, Glycosylation of the arginine vasopressin/neurophysin II common precursor, Biochem. and Bionhvs. Res. Commun., 102: 1230.CrossRefGoogle Scholar
  6. Land, H., Schutz, G., Schmale, H., and Richter, D., 1982, Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin-neurophysin II precursor, Nature, 295: 299.CrossRefGoogle Scholar
  7. Montreuil, J., 1980, Primary structure of glycoprotein glycans. Basis for the molecular biology of glycoproteins, Adv. Carbohvdr. Chem. Biochem., 37: 157.CrossRefGoogle Scholar
  8. Parish, D.C., Rodriguez, E.M., Birbeck, S.D., and Pickering, B.T., 1981, Effects of small doses of colchicine on the components of the hypothalamo-neurohypophysial system of the rat, Cell Tissue Res., 220: 809.CrossRefGoogle Scholar
  9. Pickering, B.T., 1981, The neurosecretory neuron in the 1980s, in: “Neurosecretion. Molecules, Cells, Systems”, D.S. Farner and K. Lederis, Eds., Plenum Press, New York, London.Google Scholar
  10. Rodriguez, E.M., 1971, The comparative morphology of neural lobes of species with different neurohypophysial hormones. in: 19 Memoir of the Society for Endocrinology. Eds. H. Lederis, pp. 263–291, London: Cambridge University Press.Google Scholar
  11. Rodriguez, E.M., 1971, The comparative morphology of neural lobes of Rodriguez, E.M., Peruzzo, B. and Yulis, R., 1984, Immunocytochemical evidence of the processing of neurohypophysial peptides, Proc. XV Meet. Lat. Amerc. Soc. Phvsiol. Sci., R105.Google Scholar
  12. Rodriguez, E.M., Herrera, H., Peruzzo, B., Rodriguez, S., Hein, S., and Oksche, A., 1986, Light-and electron-microscopic immunocytochemistry and lectin histochemistry of the subcommissural organ: Evidence for processing of the secretory material, Cell Tissue Res., 243: 545.CrossRefGoogle Scholar
  13. Rodriguez, E.M., Hein, S., Rodriguez, S., Herera, H., Peruzzo, B., Nualart, F., and Okschu, A., 1987, Analysis of the secretory products of the subcommissural organ. in: Functional Morphology of Neuroendocrine Systems, B. Scharrer, H. Korf, H.-G. Hartwig (eds ), Springer Verlag.Google Scholar
  14. Roth, J., 1978, The lectin: Molecular probes in cell biology and membrane research, Exn. Pathol., (Suppl.) 3: 1.Google Scholar
  15. Seidah, N.G., Benjannet, S., and Chretien, M., 1981, The complete sequence of the novel human pituitary glycopeptide. Biochem. Bionhvs. Res. Commun., 100: 901.CrossRefGoogle Scholar
  16. Smyth, D.G., and Massey, D.E., 1979, A new glycopeptide in pig ox and sheep pituitary, Biochem. Bionhvs, Res. Commun., 87: 1006.CrossRefGoogle Scholar
  17. Staneloni, R.J., and Leloir, L.F., 1982, The biosynthetic pathway of the asparagine-lined oligosaccharadies of glycoproteins. CRC Critical Review in Biochemistry, 12: 289.Google Scholar
  18. Swann, R.W., Gonzalez, C.B., Birkett, S.D., and Pickering B.T., 1983, Harbingers and hormones: inter-relationships of rat neurohypophysial hormone precursors in vivo. in: The Neurohypophysis: Structure, Function and Control, Progress in Brain Research, Vol. 60, B.A. Cross, G. Leng (eds.). Elsevier Science.Google Scholar
  19. Tasso, F., 1974, Diversite d’aspect des fibres neurosecretrices revelees dans la posthypophyse du rat par latechnique de post-fixation combinant l’osmium et le ferrocyanure de potasium. C. R. Soc. Biol. (Paris), 168: 986.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • E. M. Rodriguez
    • 1
  • B. Peruzzo
    • 1
  • L. Alfaro
    • 1
  • H. Herrera
    • 1
  1. 1.Instituto de Histologie y PatologiaUniversidad Austral de ChileValdiviaChile

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