Combined Use of Lectin Histochemistry and Immunocytochemistry for the Study of Neurosecretion
In 1971, on the occasion of a Symposium organized by Heller and Lederis, and also held in Bristol, I presented some histochemical evidence indicating that in the neural lobe of amphibians and reptilian only one of the two types of neurosecretory fibers contained carbohydrates. More substantial evidence was provided by Tasso (1974) who, working with rats at the ultrastructural level, demonstrated that glycoproteins were circumscribed to neurosecretory granules located in one of the two types of neurosecretory fibers. Later, Tasso et al., (1977), by studying the hypothalamo-neurohypophysial system of Brattleboro rats, showed that the neurosecretory granules containing glycoproteins were missing from homozygous rats and postulated that glycoproteins and arginine-vasopressin (AVP) coexisted within the same granule. Apparently, no further histochemical studies have been performed.
KeywordsSialic Acid Rough Endoplasmic Reticulum Sugar Residue Neurosecretory Granule Lectin Histochemistry
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- Gainer, H., 1981, The biology of neurosecretory neurons, in: “Neurosecretion and Brain Peptides”, J.B. Martin, S. Reichlin, and K.L. Bick, eds., Raven Press, New York.Google Scholar
- Haddad, A., Pelletier, G., Marchi, F., and Brasileiro, L., 1976, Radioautographic study of glycoprotein biosynthesis migration and renewal in the hypothalamusneurohypophysis system, Proc. 5th Colloa. Brazilian Soc. Elect. Microsc., p.62.Google Scholar
- Pickering, B.T., 1981, The neurosecretory neuron in the 1980s, in: “Neurosecretion. Molecules, Cells, Systems”, D.S. Farner and K. Lederis, Eds., Plenum Press, New York, London.Google Scholar
- Rodriguez, E.M., 1971, The comparative morphology of neural lobes of species with different neurohypophysial hormones. in: 19 Memoir of the Society for Endocrinology. Eds. H. Lederis, pp. 263–291, London: Cambridge University Press.Google Scholar
- Rodriguez, E.M., 1971, The comparative morphology of neural lobes of Rodriguez, E.M., Peruzzo, B. and Yulis, R., 1984, Immunocytochemical evidence of the processing of neurohypophysial peptides, Proc. XV Meet. Lat. Amerc. Soc. Phvsiol. Sci., R105.Google Scholar
- Rodriguez, E.M., Hein, S., Rodriguez, S., Herera, H., Peruzzo, B., Nualart, F., and Okschu, A., 1987, Analysis of the secretory products of the subcommissural organ. in: Functional Morphology of Neuroendocrine Systems, B. Scharrer, H. Korf, H.-G. Hartwig (eds ), Springer Verlag.Google Scholar
- Roth, J., 1978, The lectin: Molecular probes in cell biology and membrane research, Exn. Pathol., (Suppl.) 3: 1.Google Scholar
- Staneloni, R.J., and Leloir, L.F., 1982, The biosynthetic pathway of the asparagine-lined oligosaccharadies of glycoproteins. CRC Critical Review in Biochemistry, 12: 289.Google Scholar
- Swann, R.W., Gonzalez, C.B., Birkett, S.D., and Pickering B.T., 1983, Harbingers and hormones: inter-relationships of rat neurohypophysial hormone precursors in vivo. in: The Neurohypophysis: Structure, Function and Control, Progress in Brain Research, Vol. 60, B.A. Cross, G. Leng (eds.). Elsevier Science.Google Scholar
- Tasso, F., 1974, Diversite d’aspect des fibres neurosecretrices revelees dans la posthypophyse du rat par latechnique de post-fixation combinant l’osmium et le ferrocyanure de potasium. C. R. Soc. Biol. (Paris), 168: 986.Google Scholar