Neurosecretion pp 243-249 | Cite as

Effects of Atrial Natriuretic Polypeptide and Angiotensin II on the Supraoptic Neurons in Vitro

  • Hiroshi Yamashita
  • Kiyotoshi Inenaga


Atrial natriuretic polypeptide (ANP) was isolated from atrial tissue and exhibits powerful natriuretic, diuretic and vasodilatory activities. Its sequence has been identified and ANP-immunoreactivity has been localized not only in the atria but also in plasma and within rat hypothalamus (Tanaka et al., 1984) with dense accumulations of immunocytochemical staining in regions of the brain that are important in the control of fluid balance and cardiovascular functions (Kawata et al., 1985). In particular, ANP-immunoreactivity has been located in the anterio-ventral region of the third ventricle (AV3V). The AV3V region and the supraoptic nuclei (SON) contain numerous ANP-binding sites and there may be a functional role for ANP in the control of vasopressin release. Peripheral or central administration of atrial natriuretic factor inhibits the release of arginine vasopressin (AVP) caused by dehydration, haemorrhage and changes in plasma osmolarity (Poole et al., 1987). It is possible that ANP might antagonise the effects of AVP, so the first set of experiments were designed to investigate whether or not ANP could influence directly the activity of neurosecretory cells in the SON. The experiments were carried out in trimmed hypothalamus slice preparations and data compared with the effects of ANP on neurons in the AV3V region (Okuya & Yamashita, 1987).


Firing Rate Atrial Natriuretic Peptide Atrial Natriuretic Factor Neurosecretory Cell Supraoptic Nucleus 


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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Hiroshi Yamashita
    • 1
  • Kiyotoshi Inenaga
    • 1
  1. 1.Department of PhysiologyUniversity of Occupational and Environmental Health School of MedicineJapan Kitakyushu 807Japan

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