Advertisement

Neurosecretion pp 235-241 | Cite as

The Neonatal Rat Supraoptic Neurone in Culture:Development of a Model for Control of Peptidergic Secretion

  • P. Cobbett
  • K. Inenaga
  • P. Legendre
  • W. T. Mason

Abstract

The magnocellular neurones of the hypothalamo-neurohypophysial system have in recent years proved a valuable model in which to examine the complex control of peptidergic secretion, in particular oxytocin and vasopressin. The unique properties of the nuclei containing these neurones — principally homogeneity and high density of the neuronal population — have meant that a variety of in vivo and in vitro studies have been possible.

Keywords

Lucifer Yellow Supraoptic Nucleus Voltage Clamp Recording Phase Contrast Optic Phasic Firing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cobbett, P., and Mason, W.T., 1987, Whole cell voltage clamp recordings from cultured neurons of the supraoptic area of neonatal rat hypothalamus, Brain Res., 409: 175.CrossRefGoogle Scholar
  2. Cobbett, P., and Mason, W.T., 1988, Potential recordings from neurons of the supraoptic area of the neonatal rat hypothalamus in culture, (Submitted for publication.)Google Scholar
  3. Cobbett, P., Legendre, P., and Mason, W.T., 1987, Three types of Kt currents recorded from cultured neurones of the region of the supraoptic nucleus of the rat, J. Phvsiol., 384: 20 P.Google Scholar
  4. Dudek, F.E., and Andrew, R.D., 1985, Electrophysiological characteristics of paraventricular and supraoptic neurons. in: Vasopressin. Editor: R.W. Schrier. Raven Press New York.Google Scholar
  5. Hamill, O.P., Marty, A., Neher, E., Sakman, B., and Sigworth, E.J., 1981, Improved patch clamp techniques for high resolution current recording from cells and cell-free membrane patches, Pflugers Archiv., 391: 85.CrossRefGoogle Scholar
  6. Inenaga, K., Legendre, P., and Mason, W.T., 1987, GABA activated chloride channels in cultured neurones from the supraoptic area of neonatal rats, J. Phvsiol., 388: 12 P.Google Scholar
  7. Mason, W.T., 1980, Supraoptic neurones of rat hypothalamus are osmosensitive, Nature, 287: 154.CrossRefGoogle Scholar
  8. Mason, W.T., Cobbett, P., and Poulain, D.A., 1987 r-aminobutyric acid as an inhibitory neurotransmitter in the rat supraoptic nucleus: intracellular recordings in hypothalamic slices, Neurosci. Letters, 73: 259.Google Scholar
  9. Randle, J.C.R., Renaud, L.P., 1987, Actions of r-aminobutyric acid on rat supraoptic nucleus neurosecretory neurones in vitro, J. Phvsiol., 387: 629.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • P. Cobbett
    • 1
  • K. Inenaga
    • 1
  • P. Legendre
    • 1
  • W. T. Mason
    • 1
  1. 1.Department of NeuroendocrinologyAFRC Institute of Animal Physiology and Genetics ResearchBabraham, CambridgeUK

Personalised recommendations