Advertisement

Mapping and Analysis of Receptors for Neurohypophyseal Peptides Present in the Brain

  • E. Tribollet
  • C. Barberis
  • S. Jard
  • J. Elands
  • M. Dubois-Dauphin
  • A. Marguerat
  • J. J. Dreifuss

Abstract

Vasopressin (AVP) and oxytocin (OT) meet most of the criteria which are usually applied to assign a role as a neurotransmitter or neuromodulator to a given compound. 1) In addition to their presence in the hypothalamo-hypophysial system, they are also found in neural pathways that project to various areas of the central nervous system (Sofroniew, 1981). 2) They can be released in a calcium dependent manner from several of these extra-hypothalamic areas (Buijs, 1983). 3) They affect the electrical activity of single neurones (Miihlethaler et al., 1985), as well as integrative processes such as temperature control, blood pressure regulation, avoidance behavior, etc. (Kovacs et al., 1987). The commercial availability of tritium-labelled AVP and OT has allowed the detection of high affinity binding sites for these peptides in the brain (Audigier and Barberis, 1985; Jard et al., 1987; Van Leeuwen, 1987). The experimental data presented in this chapter show the type of information which can be obtained by in vitro light microscopic autoradiography. Four points are considered. First, the distribution of high affinity binding sites is described for the rat and the guinea-pig brain; surprisingly, marked differences in their location apparently exist between these two species. Second, brain AVP and OT binding sites in the rat brain are compared to peripheral receptors, using synthetic structural analogues with enhanced selectivity for OT receptors and for the known subtypes of AVP receptors. Third, a case of long term receptor regulation is described, namely the effects of castration and of gonadal steroids on the density of [H]OT binding in the rat brain. Finally, preliminary results obtained with a newly synthetized and iodinated OT receptor ligand are presented and compared with those obtained with tritiated oxytocin.

Keywords

Stria Terminalis Amygdaloid Nucleus High Affinity Binding Site Anterior Olfactory Nucleus External Plexiform Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Audigier, S. and Barberis, C., 1985, Pharmacological characterization of two specific binding sites for neurohypophyseal hormones in hippocampal synaptic membranes of the rat, EMBO J., 4: 665.Google Scholar
  2. Buijs, R.M., 1983, Vasopressin and oxytocin. Their role in neurotransmission, Pharmacol. Ther., 22: 127.Google Scholar
  3. Caldwell, J.D., Prange, A.J., Jr., and Petersen C. A., 1986, Oxytocin facilitates the sexual receptivity of estrogen-treated female rats, Neuropeptides, 7: 175.CrossRefGoogle Scholar
  4. De Kloet, E. R., Rotteveel, F., Voorhuis, Th. D. and Terlou, M., 1985, Topography of binding sites for neurohypophyseal hormones in rat brain, Eur. J. Pharmacol., 110: 113.CrossRefGoogle Scholar
  5. De Kloet, E. R., Voorhuis, Th. D., Boschma, Y. and Elands, J., 1986, Estradiol modulates density of putative ‘oxytocin receptors’ in discrete rat brain regions, Neuroendocrinologv, 44: 415.CrossRefGoogle Scholar
  6. Elands, J., Barberis, C., Jard, S., T liiipllet, E., Dreifuss, J. J., Bankowsk, K., Manping, M. and Sawyer, W. H., 1987, [IJ-labelled d(CH2)5[Tyr(Me)2,Thr,Tyr-NH2y]OVT: a selective OT receptor ligand, Eur. J. Pharmacol., (In apress).Google Scholar
  7. Fahrbach, S. E., Morel!, J. L. and Pfaff, D. W., 1985, Possible role for endogenous oxytocin in estrogen-facilitated maternal behavior in rats. Neuroendocrinoloav 40: 526.CrossRefGoogle Scholar
  8. Freund-Mercier, M. J., Stoeckel, M. E., Palacios, J. M., Paws, A., Reichart, J. M., Porte, A. and Richard, Ph., 1987, Pharmacological characteristics and anatomical distribution of [3H]oxytocin-binding sites in the Wistar rat brain studied by autoradiography, Neuroscience, 20: 599.CrossRefGoogle Scholar
  9. Jard, S., 1983, Vasopressin isoreceptors in mammals: relations to cyclic AMP-dependent and cyclic AMP-independent mechanisms, in: A. Kleinzeller (ed.), Membrane Receptors, Current Topics in Membranes and Transport, Vol. 18, Academic Press, New York, pp. 225–285.Google Scholar
  10. Jard, S., Barberis, C., Audigier, S. and Tribollet, E., 1987, Neurohypophyseal hormone receptor systems in brain and periphery, in E. R. De Kloet, V. M. Wiegart and D. de Wied (eds.), Neuropeptides and Brain Function, Progress in Brain Research, Vol. 72, Elsevier, Amsterdam, pp. 173–187.CrossRefGoogle Scholar
  11. Kovacs, G. L., Szabo, Gy., Sastryal, Z. and Telegdy Gy., 1987, Neurohypophyseal hormones and behavior, in: E. R. De Kloet, V. M. Wiegart and D. de Wied (eds.), Neuropeptides and Brain Function, Progress in Brain Research, Vol. 72, Elsevier, Amsterdam, pp. 109–118.CrossRefGoogle Scholar
  12. Mühlethaler, M., Raggenbass, M. and Dreifuss, J. J., 1985, Oxytocin and vasopressin. in: M. A. Rogawski and J. L. Barker (eds.), Neurotransmitter Actions in the Vertebrate Nervous System, Raven Press, New York, pp. 431–450.Google Scholar
  13. Raggenbass, M., Dubois-Dauphin, M., Charpak, S. and Dreifuss, J. J., 1987, Neurons in the dorsal motor nucleus of the vagus nerve are excited by oxytocin in the rat but not in the guinea-pig, Proc. Natl. Acad. Sci. USA., 84: 3926.CrossRefGoogle Scholar
  14. Sofroniew, M. V., 1985, Vasopressin, oxytocin and their related neurophysins. in:A. Björklund and T. Hökfelt (eds.), GABA and Neuropeptides in the CNS, Handbook of Chemical Neuroanatomy, Vol. 4, Elsevier, Amsterdam, pp. 93–165.Google Scholar
  15. Tribollet, E., Barberis, C., Jard, S., Dubois-Dauphin, M. and Dreifuss, J. J., 1987, Localization and pharmacological characterization of high affinity binding sites for vasopressin and oxytocin in the rat brain by light microscopic autoradiography, Brain Research, (In apress).Google Scholar
  16. Van Leeuwen, F. W., 1987, Vasopressin receptors in the brain and pituitary. in: D. M. Gash and G. J. Boer (eds.), Vasopressin: Principles and Properties, Plenum Press, New York, pp. 477–496.Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • E. Tribollet
    • 1
  • C. Barberis
    • 2
  • S. Jard
    • 2
  • J. Elands
    • 2
  • M. Dubois-Dauphin
    • 1
  • A. Marguerat
    • 1
  • J. J. Dreifuss
    • 1
  1. 1.Department of PhysiologyUniversity Medical CenterGeneva 4Switzerland
  2. 2.CNRS-INSERM Center of Pharmacology EndocrinologyMontpellierFrance

Personalised recommendations