Cytoplasmic Organisation and the Properties of Cell Water: Speculations on Animal Cell Cryopreservation

  • J. S. Clegg
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 147)


The idea that the aqueous regions of cytoplasm consist of a concentrated and chaotic solution or proteins (and other macromolecules), coenzymes and metabolites of various kinds, and inorganic ions has been with us for a long time and still seems to be the commonly accepted paradigm. During the last 10 years, however, evidence has been steadily accumulating which suggests that the situation is far more complicated and interesting. In this brief essay I will summarise some of the principal reasons to believe that cytoplasmic organisation may extend to the level of individual macromolecules, and that the “crowded solution paradigm” is largely an assumption based upon cell disruption metholodolgy. Mention will also be made of the potential importance of cytoplasmic organisation in determining the physical properties of intracellular water. Both matters will be considered briefly in the context of animal cell cryopreservation.


Electron Spin Resonance Glycolytic Enzyme Intracellular Water Cell Water Euglena Gracilis 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • J. S. Clegg
    • 1
  1. 1.Bodega Marine LaboratoryUniversity of CaliforniaBodega BayUSA

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