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Cytoplasmic Organisation and the Properties of Cell Water: Speculations on Animal Cell Cryopreservation

  • J. S. Clegg
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 147)

Abstract

The idea that the aqueous regions of cytoplasm consist of a concentrated and chaotic solution or proteins (and other macromolecules), coenzymes and metabolites of various kinds, and inorganic ions has been with us for a long time and still seems to be the commonly accepted paradigm. During the last 10 years, however, evidence has been steadily accumulating which suggests that the situation is far more complicated and interesting. In this brief essay I will summarise some of the principal reasons to believe that cytoplasmic organisation may extend to the level of individual macromolecules, and that the “crowded solution paradigm” is largely an assumption based upon cell disruption metholodolgy. Mention will also be made of the potential importance of cytoplasmic organisation in determining the physical properties of intracellular water. Both matters will be considered briefly in the context of animal cell cryopreservation.

Keywords

Electron Spin Resonance Glycolytic Enzyme Intracellular Water Cell Water Euglena Gracilis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Wolosewick, J.J. and Porter, K.R., Microtrabecular lattice of the cytoplasmic ground substance: artifact or reality? J. Cell Biol. 82:114, (1979).PubMedCrossRefGoogle Scholar
  2. 2.
    Porter, K.R., Berkerle, M. and McNiven, A., The cytoplasmic matrix, Mod. Cell Biol. 2:259 (1983).Google Scholar
  3. 3.
    Porter, K.R., Structural organisation of the cytomatrix, in: Organization of Cell Metabolism, G.R. Welch, J.S. Clegg, eds, Plenum Press, New York, pp 9 (1987).Google Scholar
  4. 4.
    Kondo, H., What is the microtrabecula? J. Electron Microsc. 34:123 (1985).Google Scholar
  5. 5.
    Bridgeman, P.C. and Reese, T.S., The structure of cytoplasm in directly frozen cultured cells. I. Filamentous meshworks and the cytoplasmic ground substance, J. Cell Biol. 99:1655 (1984).CrossRefGoogle Scholar
  6. 6.
    Wojcieszyn, J.W., Schlegel, R.A. and Jacobson K.A., Measurements of the diffusion of macromolecules injected into the cytoplasm of living cells, Cold Spring Harbour Symp. 46:39 (1981).CrossRefGoogle Scholar
  7. 7.
    Luby-Phelps, K., Taylor, D.L. and Lanni, F., Probing the structure of cytoplasm, J. Cell Biol. 102:2015 (1986).PubMedCrossRefGoogle Scholar
  8. 8.
    Paine, P.L. Diffusive and nondiffusive proteins in vivo, J. Cell Biol. 99:188s (1984).PubMedCrossRefGoogle Scholar
  9. 9.
    Fulton, A.B., How crowded is the cytoplasm? Cell 30:345 (1982).PubMedCrossRefGoogle Scholar
  10. 10.
    Dabauvalle, M.C and Franke, W.W. Determination of the intracellular state of soluble macromolecules by gel filtration in vivo in the cytoplasm of amphibian oocytes, J. Cell Biol. 102:2006 (1986).PubMedCrossRefGoogle Scholar
  11. 11.
    Mastro, A.M. and Hurley, D.J., Diffusion of a small molecule in the aqueous compartments of mammlian cells, in: Organisation of Cell Metabolism, G.R. Welch, and J.S. Clegg, eds., Plenum Press, New York, pp 57 (1987).Google Scholar
  12. 12.
    Schliwa, M., van Blerkom, J. and Porter, K.R., Stabilization of the cytoplasmic ground substance in detergent opened cells, Proc. Nat. Acad. Sci. 78:4329 (1981).PubMedCrossRefGoogle Scholar
  13. 13.
    Knull, H.R., Glycolytic enzyme-cytomatrix intractions, in: Molecular Mechanisms in the Regulation of Cell Behaviour, C. Weymouth, ed. Alan R. Liss, Inc., New York (1987) in press.Google Scholar
  14. 14.
    Clarke, F.M., Morton, D.J., Stephan, P. and Wiedemann, J. The functional duality of glycolytic enzymes: potential integrators of cytoplasmic structure and function, in: Cell Motility: Mechanism and Regulation, H. Ishikawa, S. Hatano, and H. Sato, eds., University of Tokyo Press, Tokyo. 235 (1985).Google Scholar
  15. 15.
    Morton, D.J., Wiedemann, J.F., Clarke, F.M, Stephan, R. and Stewart, M. A cytoskeletal role for glycolytic enzymes, Micron 13:377 (1982).Google Scholar
  16. 16.
    Masters, C.J., Interactions between glycolytic enzymes and components of the cytomatrix, J. Cell Biol. 99:222s (1984).PubMedCrossRefGoogle Scholar
  17. 17.
    Stephan, P., Clarke, F. and Morton, D., The indirect binding of triose-phosphate isomerase to myofibrils to form a glycolytic enzyme mini-complex, Biochim. Biophys. Acta. 873:127 C (1986).PubMedCrossRefGoogle Scholar
  18. 18.
    Mansell, J.L. and Clegg, J.S. Cellular and molecular consequences of reduced cell water content, Cryobiology 20:591 (1983).PubMedCrossRefGoogle Scholar
  19. 19.
    Clegg, J.S. and Gordon, E.P. Respiratory metabolism of L-929 cells at different water contents and volumes, J. Cell. Physiol. 124:299, (1985).PubMedCrossRefGoogle Scholar
  20. 20.
    Clegg, J.S., Properties and metabolism of the aqueous cytoplasm and its boundaries, Amer. J. Physiol. 246:R133, (1984).Google Scholar
  21. 21.
    Clegg, J.S., On the internal environment of animal cells, in: Microcompartmentation, D. Jones, ed. CRC Press, Ft. Lauderdale, Florida (1987) in press.Google Scholar
  22. 22.
    Kempner, E.S. and Miller, J.H. The molecular biology of Euglena gracilis IV. Cellular stratification by centrifuging, Exp. Cell Res., 51:141 (1968).PubMedCrossRefGoogle Scholar
  23. 23.
    Kempner, E.S. and Miller, J.H., The molecular biology of Euglena gracilis V. Enzyme localisation, Exp. Cell Res. 51:150 (1968).PubMedCrossRefGoogle Scholar
  24. 24.
    Welch, G.R., ed, Organised Multienzyme Systems, Academic Press, New York, pp 458 (1985).Google Scholar
  25. 25.
    Welch, G.R. and Clegg, J.S., eds, Organisation of Cell Metabolism, Plenum Press, New York, pp 389 (1987).Google Scholar
  26. 26.
    Bhargava, P., Is the “soluble” phase of cells structured? Biosystems 18:135 (1985).PubMedCrossRefGoogle Scholar
  27. 27.
    Srivastava, D.K. and Bernhard, S.A. Enzyme-enzyme interactions and the regulation of metabolic reaction pathways, Curr. Top. Cell. Reg. 28:1 (1986).Google Scholar
  28. 28.
    Srere, PA., Complexes of sequential metabolic enzymes, Ann. Rev. Biochem, 56:21 (1987).CrossRefGoogle Scholar
  29. 29.
    Clegg, J.S., On the physical properties and potential roles of intracellular water, in: Organisation of Cell Metabolism, G.R. Welch and J.S. Clegg, eds., Plenum Press, New York, pp 41 (1987).Google Scholar
  30. 30.
    Ling, G.N. In Search of the Physical Basis of Life, Plenum Press, New York,, pp 791 (1984).CrossRefGoogle Scholar
  31. 31.
    Pullman, A., Vasilescu, V. and Packer, L., Eds., Water and Ions in Biological Systems, Plenum Press, New York,, pp 508 (1985).Google Scholar
  32. 32.
    Franks, F. and Mathias S., Eds., Biophysics of Water, Wiley, New York, pp 440, (1982).Google Scholar
  33. 33.
    Gershon, N.D., Porter, K.R. and Trus, B.L., The cytoplasmic matrix: its volume, surface area, and the diffusion of molecules throgh it, Proc. Nat. Acad. Sci 82:5030 (1985).PubMedCrossRefGoogle Scholar
  34. 34.
    Parsegian, V.A. and Rau, D.C., Water near intracellular surfaces, J. Cell Biol. 99:196s, (1984).PubMedCrossRefGoogle Scholar
  35. 35.
    Evans, D.F., and Ninham, B.W., Molecular forces in self-organisation of amphiphiles, J. Phy. Chem. 90:226 (1986).CrossRefGoogle Scholar
  36. 36.
    Pashley, R. and Israelachvilli, J.N., Molecular layering of water in thin films between mica surfaces and its relation to hydration forces, J. Coll. Interf. Sci. 101:511 (1984).CrossRefGoogle Scholar
  37. 1.
    J.L. Finney, Hydration in protein crystals, in: Water: A Comprehensive Treatise, (ed. P. Franks), Plenum, New York 6:47 (1979).Google Scholar
  38. 2.
    G.N. Ling and M.M. Ochsenfeld, Studies on the physical state of water in living cells and model systems. I. The quantitative relationship between the concentration of gelatin and certain oxygen-containing polymers and their influence upon the solubility of water and Na+ salts. Physiol. Chem. Phys. and Med. NMR 15:127 (1983).Google Scholar
  39. 3.
    P.A. Albertsson, Partition of cell particles and macromolecules, Third edition, Wiley-Interscience, New York, 1 (1986).Google Scholar
  40. 4.
    K.R. Porter and J.B. Tucker, The ground substance of the living cell Scientific American 244:56 (1981).PubMedCrossRefGoogle Scholar
  41. 5.
    A.M. Mastro and D.J. Hurley, Diffusion of a small molecule in the aqueous compartments of mammalian cells, in: The organisation of Cell Metabolism (eds. G.R. Welch and J.S. Clegg), Plenum, New York, 57 (1987).Google Scholar
  42. 6.
    K. Luby-Phelps, P. Castle, D.L. Taylor and F. Lanni, Further evidence for the existence of a structural network in the cytoplasmic ground substance of living cells, J. Cell Biol. 103:286a (1986).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • J. S. Clegg
    • 1
  1. 1.Bodega Marine LaboratoryUniversity of CaliforniaBodega BayUSA

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