Evaluation of Biochemical Processes by NMR
Phosphorus nuclear magnetic resonance (31P NMR) is finding increasing application as a non-invasive method of studying metabolism in living systems. 31P spectra include signals from ATP, phosphocreatine (in muscle and brain), and inorganic phosphate, and in addition to monitoring the relative concentrations of these metabolites, it is also possible to measure the intracellular pH. The technique is therefore ideally suited to examining the changes in high energy phosphates and pH that are associated with periods of ischaemia, anoxia, or hypoxia.
KeywordsCold Storage Rabbit Heart Cardioplegic Solution Preservation Solution High Energy Phosphate
Unable to display preview. Download preview PDF.
- 1.M. Bernard, P. Menasche, P. Canioni, E. Fontanarava, C. Grousset, A. Piwnica and P. Cozzone, Influence of the pH of cardioplegia solutions on intracellular pH, high-energy phosphates, and postarrest performance. Protective effects of acidotic, glutamate-containing cardioplegic perfusates, J. Thorac. Cardiovasc. Surg. 90:235 (1985).PubMedGoogle Scholar
- 4.D.G. Gadian, “Nuclear magnetic resonance and its applications to living systems”, Oxford University Press, Oxford (1982).Google Scholar
- 5.T.A.H. English, D.K.C. Cooper. R. Medd, R. Walton and D. Wheeldon, Orthotopic heart transplantation after 16 hours of ischaemia, Proc. Eur. Soc. Artificial Organs 6:340 (1979).Google Scholar
- 12.S. Darracott-Cankovic, D. Wheeldon, R. Cory-Pearce, J. Wallwork and T.A.H. English, Biopsy assessment of 50 hearts during transplantation, J. Thorac. Cardiovasc. Surg. In Press.Google Scholar
- 15.D.E. Pegg, Principles of tissue preservation, In: “Progress in Transplantation”, P.J. Morris and N.L. Tilney, eds., Churchill Livingstone (1985).Google Scholar
- 17.T.A. English, J. Foreman, D.G. Gadian, D.E. Pegg, D. Wheeldon and S.R. Williams, Three solutions for preservation of the rabbit heart at 0°C: A comparison using 31P nuclear magnetic resoaance spectroscopy, Submitted to J. Thorac. Cardiovasc. Surg.Google Scholar
- 18.R.Y. Calne, Liver, In: “Organ preservation for transplantation”, A. Karow and D. Pegg, eds., Marcel Dekker, New York (1981).Google Scholar
- 20.M. Ukikusa and T. Lie, Significance of alkaline pH in liver preservation, Cryobiology 20:733 (1983).Google Scholar
- 22.B.J. Fuller, A.L. Busza, E. Proctor, M. Myles, D.G. Gadian and K.E.F. Hobbs, Control of pH during hypothermic liver storage: What role for the storage solution? Transplantation, In press.Google Scholar