An Introduction to the Problems of Organ Cryopreservation
Attempts to freeze whole organs such as kidneys by the application of techniques similar to those used for the cryopreservation of cells in suspension have been unsuccessful, although very few exceptions have been reported (1,2,3,4). There may be a number of reasons for this: not only does the freezing of organs involve the relatively well known problems of cell cryopreservation, but inherent properties of intact organs add further difficulties of a nature less well understood. There seems little doubt, however, that a future protocol for organ cryopreservation must include methods for permeation of the organ with a cryoprotective agent (CPA) and for controlled cooling and warming at rates which will permit the survival of individual cells.
KeywordsOrgan Preservation Osmotic Effect Rabbit Kidney Vehicle Solution Cryopreservation Protocol
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