The Design of an Electromagnetic Rewarming System for Cryopreserved Tissue

  • T. P. Marsland
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 147)


Techniques for the cryopreservation of cells are well developed for many cell types (1) however, a successful, reproducible cryopreservation protocol has yet to be found for whole organs. A clear feature of the many cryopreservation experiments reported in the literature is the importance of using appropriate cooling and warming rates for a particular biological system. Although there are many complex and interacting factors in organ cryopreservation, experimental evidence from a range of cell and organ model systems (2,3) suggest that warming should be much more rapid than the 1–2°C/min attainable with thermal conduction. Also, rapid warming is likely to be an essential component of any method that might be developed for organ cryopreservation using vitrification (4). In fact, the possible benefits of such rapid warming rates have not been thoroughly investigated in large organ systems, largely because of the difficulties associated with producing such high warming rates in a controlled manner. This chapter is concerned with using electromagnetic (EM) fields at radio or microwave frequencies to address this problem.


Dielectric Property Dielectric Loss High Warming Rate Effective Conductivity Power Deposition 


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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • T. P. Marsland
    • 1
  1. 1.MRC Medical Cryobiology GroupUniversity Dept. of SurgeryCambridgeUK

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