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Enzyme Immunoassay of Plant Constituents

  • Richard L. Mansell
  • Cecilia A. McIntosh

Abstract

The use of immunoassay to quantitate plant derived compounds was first developed in clinical laboratories in the early 1970’s. These first assays were directed toward small organic molecules, e. g., colchicine (Boudene et al., 1975), nicotine (Langone et al., 1973), and morphine (Spector, 1971), which have medicinal or pharmaceutical value. These assays were based on the radioimmunoassay (RIA) and were developed for compounds whose small molecular size renders them non-immunogenic. Assays for low molecular weight compounds of animal origin had been developed earlier (see Erlanger, 1980, and references therein) using synthesized protein-hapten conjugates for immunization. The immunoassays mentioned above demonstrated that the hapten-protein conjugate principle (Landsteiner, 1945) also could be used to induce animals to produce antibodies against compounds of plant origin.

Keywords

Abscisic Acid Soya Protein Enzyme Immunoassay Antibody Preparation Lysergic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Richard L. Mansell
    • 1
  • Cecilia A. McIntosh
    • 1
  1. 1.Biology DepartmentUniversity of South FloridaTampaUSA

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