Enzyme Immunoassay Based on Partition Affinity Ligand Assay (PALA) System
A crucial step in most immunoassays is the separation of free from bound reactant. The assays based on solid phase technology which were developed to overcome this problem are characterized by elaborate washing procedures. In order to avoid such steps and make immunoassays easier to perform, certain homogeneous assays have been developed (Ullman and Maggio 1980). The present paper deals with yet another approach to immunoassays where the binding takes place in free solution and separation is achieved by an extraction step using an aqueous two-phase system (Mattiasson 1983). The assay procedure may be designed in such a way that all steps are carried out in one and the same vessel.
KeywordsBinding Assay Phase System Bottom Phase Competitive Binding Assay Competitive Assay
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