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Chromogenic Substrates for Enzyme Immunoassay

  • Chapter
Nonisotopic Immunoassay

Abstract

Out of a variety of enzymes which have been described according to their kinetics and structure only a few have turned out to be marker enzymes in the enzyme immunoassay (EIA). The ones that are mostly used are horseradish peroxidase (HRP), calf intestinal alkaline phosphatase (AP) and ß-galactosidase from E. coli (ßGal). The suitability of enzymes as markers is not only determined by their great molar activity that is to be largely maintained even after binding to immunoreactants, their stability and commercial availability but especially by their simple, practicable and sensitive detectability.

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Author information

Authors and Affiliations

  1. Institute of Pathological and Clinical Biochemistry, Germany

    Bärbel Porstmann & Tomas Porstmann

  2. Institute of Medical Immunology, Faculty of Medicine (Charite), Humboldt University, Berlin, GDR

    Bärbel Porstmann & Tomas Porstmann

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  1. Bärbel Porstmann
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  2. Tomas Porstmann
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Editors and Affiliations

  1. University of California, Irvine, Irvine, California, USA

    T. T. Ngo

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© 1988 Plenum Press, New York

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Porstmann, B., Porstmann, T. (1988). Chromogenic Substrates for Enzyme Immunoassay. In: Ngo, T.T. (eds) Nonisotopic Immunoassay. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5466-6_3

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  • DOI: https://doi.org/10.1007/978-1-4684-5466-6_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5468-0

  • Online ISBN: 978-1-4684-5466-6

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