Nephelometric Inhibition Immunoassay for Small Molecules
The use of nephelometry, and especially rate nephelometry (Tiffany, Parella, Johnson and Burtis, 1974), for the measurement of specific serum proteins has become very well established. In nephelometric methods, the marked increases in light scattering which occur as a consequence of the immunochemical reaction between polyvalent antigens (such as proteins) and their antibodies is utilized. Thus, while solutions of protein molecules scatter only a small to moderate amount of visible light, their reactions with their specific antisera lead to the formation of crosslinked three dimensional immuno complexes large enough to scatter a significantly increased amount of light, and, eventually, to precipitate. In rate nephelometry, the rate of increase of light scattering during the course of this reaction is utilized to provide a measure of the protein concentration.
KeywordsRate Signal Antibody Dilution Antibody Molecule High Rate Signal Immunochemical Reaction
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