Interface Immunoassays Using the Evanescent Wave

  • Ranald Sutherland
  • Claus Dähne
  • Rudolf Slovacek
  • Barry Bluestein


The use of the evanescent wave to detect fluoro-immunoassay reactions at an interface is reviewed. Two systems are discussed in detail as practical examples of applying the evanescent wave detection of immunoassays. These systems are based on light guides of different geometry, a planar quartz slide and a quartz optical fibre. Both systems are based on attaching antibodies covalently to the waveguide surface and using fluoresceine as the label. Examples are given of measuring human IgG and human albumin by two-site immunofluorometric assay with the slide waveguide, and ferritin using the fibre optic. An example is also given of measuring digoxin by a limited reagent assay using the optical fibre. The future application of evanescent wave immunoassay is analyzed in the discussion.


Internal Reflection Evanescent Wave Planar Waveguide Total Internal Reflection Fluorescence Multiple Internal Reflection 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Ranald Sutherland
    • 1
  • Claus Dähne
    • 1
  • Rudolf Slovacek
    • 2
  • Barry Bluestein
    • 2
  1. 1.Biomedical GroupBattelle InstituteGenevaSwitzerland
  2. 2.Biomedical Research LaboratoriesCiba Corning Diagnostics Corp.CambridgeUSA

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