Methods for Enzyme-Labeling of Antigens Antibodies and their Fragments

  • Eiji Ishikawa
  • Seiichi Hashida
  • Takeyuki Kohno
  • Koichiro Tanaka


A number of reagents have been used for enzyme-labeling of antigens and antibodies during the past two decades. However, most of them suffer from serious disadvantages, and only a few reagents are in current use, including glutaraldehyde, periodate, maleimide compounds and pyridyl disulfide compounds (Ishikawa et al., 1983a).


Sodium Phosphate Buffer Pepsin Digestion Conjugation Efficiency Maleimide Group Calf Intestine 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Eiji Ishikawa
    • 1
  • Seiichi Hashida
    • 1
  • Takeyuki Kohno
    • 1
  • Koichiro Tanaka
    • 1
  1. 1.Department of BiochemistryMedical College of MiyazakiKiyotake, MiyazakiJapan

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