Reflectance Method for Immunoassay on Solid Surfaces

  • Hans Arwin
  • Stefan Welin
  • Ingemar Lundström


In a solid phase immunoassay, one specifically wants to measure antibody adsorption from a liquid phase onto an antigen covered surface or vice versa. The objective is therefore to characterize and quantify adsorbed organic material on a solid substrate surface. Quantification is the most important task from a clinical point of view. We can either use the rate of adsorption or the total adsorbed amount of material as data. Most methods for quantification of binding reactions on solid surfaces, e. g. radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA) (Engwall and Pesce, 1978), do not permit continuous (kinetic) measurement of binding reactions. In clinical studies one is therefore confined to the type of measurements in which the reaction under study either is terminated after a certain time or alternatively allowed to run to an equilibrium. In this communication we will refer to this type of measurements as steadystate measurements, although it is not always an equilibrium situation we study. We will here describe a simple optical technique by means of which it is possible to do either kinetic or steady-state measurements.


Silicon Surface Oxide Thickness Reflectance Technique Reflectance Change Biomedical Analysis 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Hans Arwin
    • 1
  • Stefan Welin
    • 1
  • Ingemar Lundström
    • 1
  1. 1.Laboratory of Applied Physics, Department of Physics and Measurement TechnologyLinköping Institute of TechnologyLinköpingSweden

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