A Primer for Multilayer Immunoassay

  • Carl M. Berke


The forces shaping evolution of medical diagnostic technology today have created a growing demand for what is broadly termed “dry chemistry”. The success of dipstick products derived from paper impregnated reagents has stimulated the development of a new generation of chemistry delivery systems based on a multilayer format. The principal attraction of multilayer formats is the potential capability for integration of sequential reaction chemistries thereby transforming a complex protocol to one that is “user-friendly”, with less reliance on analyst skill or capital-intensive automation. Such a format change may be likened to the development of instant color photographic materials whereby each manual operation of the process along with its specific set of associated reagents, is reduced to a separate thin layer. The reagent layers can be maintained segregated and inactive until initiated by the application of sample fluid. The net effect is to transform operational complexity into media complexity. Just as the photographic laboratory has been elegantly embodied in a sheet of Polaroid self-developing film, so have classical clinical chemistries been translated to a slide format, as magnificently demonstrated by the Ektachem system developed at Eastman Kodak (Curme, 1978; Shirey, 1983).


Glycidyl Methacrylate Detection Zone Detection Layer Spreader Layer Multilayer Format 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Carl M. Berke
    • 1
  1. 1.Hygeia SciencesNewtonUSA

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