Separation-Required Chemiluminescence Immunoassays for Steroids

  • F. Kohen
  • Y. Ausher
  • S. Gilad
  • J. De Boever
  • G. J. R. Barnard
  • J. B. Kim


Recent studies by Schroeder et al. (1978), from our laboratory (Kohen et al., 1985) and elsewhere (Pratt et al., 1978) have indicated that chemiluminescence immunoassay can be a feasible alternative to radioimmunoassay (RIA) of haptens. For our studies we chose as a chemiluminescent marker, derivatives of isoluminol possessing alkyl chains of an optional length of 2–6 atoms and terminating with a primary amino group (Schroeder and Yeager, 1978). The advantages of these compounds are that they can be measured at the level of pmol (Schroeder and Yeager, 1978), are stable (Pazzagli et al., 1983) and can be conjugated easily to carboxy derivatives of steroid or steroid glucuronides (Kohen et al., 1983) to yield the corresponding steroid-chemiluminescent tagged conjugates which can be utilized as markers in immunoassay procedures. Using these markers, we adopted two types of formats: (a) assays that do not require physical separation of bound and free hormone, so called “homogeneous”, and (b) assays that require a separation step, so called “heterogeneous”.


Menstrual Cycle Chemiluminescence Immunoassay Urinary Steroid Early Morning Urine Immunoassay Procedure 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • F. Kohen
    • 1
  • Y. Ausher
    • 1
  • S. Gilad
    • 1
  • J. De Boever
    • 2
  • G. J. R. Barnard
    • 3
  • J. B. Kim
    • 4
  1. 1.Dept. of Hormone ResearchWeizmann Institute of ScienceRehovotIsrael
  2. 2.Akademisch ZiekenhuisVrouwenkliniek/Poli IIIGentBelgium
  3. 3.Dept. of Obs./Gyn.King’s Coll. Sch. of Med. and Dent.LondonUK
  4. 4.Col. of Animal Husb.Kon-Kuk Univ.SeoulKorea

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