Advertisement

Solid-Phase Luminescence Immunoassays Using Kinase and Aryl Hydrazide Labels

  • William G. Wood
  • Harald Fricke
  • Christian J. Strasburger

Abstract

Although bioluminescence has been known for over 3000 years, and chemiluminescence for over a century, (Radziszewski, 1877), it is only recently that these phenomena have been adapted for routine, in-vitro diagnosis. This has partly been due to the lack of suitable measuring equipment, and partly to the lack of robust immunoassay systems.

Keywords

Light Output Microcrystalline Cellulose Acid Hydrazide Succinic Anhydride Alkaline Peroxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Braun, J., Schultek, T., Tegtmeier, K.F., Florenz, A., Rohde, C., Wood, W.G. (1986), Luminometric assays of seven acute phase proteins in minimal volumes of serum, sputum, and bronchioalveolar lavage. Clin. Chem. In Print.Google Scholar
  2. Brolin, S.E., Borglund, E., Tegner, L., Wettermark, G. (1971), Photokinetic microassay based on dehydrogenase reactions and bacterial luciferase. Anal. Biochem., 42, 124–135PubMedCrossRefGoogle Scholar
  3. Caldini, A.L., Orlando, C., Barni, T., Messeri, G., Pazzagli, M., Baldi, E., Serio, M. (1986), Measurement of human seminal plasma transferrin by a chemiluminescent method. Clin. Chem. In PrintGoogle Scholar
  4. Carrico, R.J., Yeung, K.K., Schroeder, H.R., Buckler, R.T., Christner, J.E. (1976), Specific protein binding reactions with ligand-ATP conjugates and firefly luciferase. Anal. Biochem., 16, 95–110CrossRefGoogle Scholar
  5. Ekins, R.P. (1978), Quality control and assay design. In: Radioimmunoassay and related procedures in medicine 1977, IAEA, Vienna, Vol II, pp 39–56Google Scholar
  6. Fricke, H., Strasburger, C.J. Wood, W.G. (1982), Enzyme enhanced luminescence immunoassay for the determination of transferrin in serum J. Clin. Chem. Clin. Biochern. 20, 91–94Google Scholar
  7. Fricke, H. (1986), Entwicklung biolumineszenzimmunologischer Testverfahren als mögliche Alternative zum Radioimmunoassay. Inauguraldissertation zur Erlangen der Doktorwurde, Medizinische Universität zu Lübeck, 107 pages.Google Scholar
  8. Gadow, A., Fricke, H., Strasburger, C.J., Wood, W.G. (1984), Synthesis and evaluation of luminescent tracers and hapten-protein conjugates for use in luminescent immunoassays with immobilised antibodies and antigens. Part II of a critical study of macro solid phases for use in immunoassay systems. J. Clin. Chem. Clin. Biochem. 22, 337–347PubMedGoogle Scholar
  9. Gundermann, K.D. (1968), Chemilumineszenz organischer Verbindungen — Ergebnisse und Probleme (Hrsg. Broderek, H., Hafner, K., Müller, E.), Springer, Berlin, Heidelberg, New York, 174 pages.Google Scholar
  10. Hersch, L.S., Vann, W.F., Wilhelm, S.A. (1979), A luminol-assisted competitive binding immunoassay of human immunoglobulin G. Anal. Biochem. 93, 267–271CrossRefGoogle Scholar
  11. Hunter, W.M. (1982), Recent advances in radioimmunoassay and related procedures. In: Radioimmunoassay and related procedures in medicine 1982, IAEA, Vienna, pp 3–21.Google Scholar
  12. Radziszewski, B. (1877), Untersuchungen über Hydrobenzamid, Amarin und Lophin. Ber. Chem. Ges. 10, 70–75CrossRefGoogle Scholar
  13. Schroeder, H.R., Vogelhut, P.O., Carrico, R.J., Boguslaski, R.C., Buckler, R.T. (1976), Competitive protein binding assay for biotin monitored by chemiluminescence. Anal. Biochem. 48, 1933–1937Google Scholar
  14. Shapiro, R., Chan, J., Pierson, A., Vaccaro, K., Quick, J. (1984), Protein-enhanced fluorescein chemiluminescence used in an immunoassay for rubella antibody in serum. Clin. Chem. 30, 889–893PubMedGoogle Scholar
  15. Tsuji, A., Maeda, M., Arakawa, H. (1984), Enzyme immunoassay monitored by chemiluminescence reaction using bis(2,4,6-trichlorophenyl) oxalate fluorescent dye. In: Procedings of the third international symposium on analytical applications of bioluminescence and chemiluminescence, Birmingham, U.K. Eds. Kricka, L.J., Stanley, P.E., Thorpe, G.H.G., Whitehead, T.P., Academic Press, London, Orlando, pp 253–256.Google Scholar
  16. Weeks, I., Beheshti, I., McCapra, F., Campbell, A.K., Woodhead, J.S. (1983), Acridinium esters as high-specific-activity labels in immunoassay. Clin. Chem. 29, 1474–1479PubMedGoogle Scholar
  17. Weeks, I., Sturgess, M., Siddle, K., Jones, M.K., Woodhead, J.S. (1984), A high sensitivity immunochemiluminometric assay for human thyrotropin. Clin. Endocrinol. 20, 489–495CrossRefGoogle Scholar
  18. Whitehead, T.P., Thorpe, G.H.G., Carter, T.J.N., Groucott, C., Kricka, L.J. (1983), Enhanced luminescence procedure for sensitive determination of peroxide labelled conjugates in immunoassay. Nature 305, 158–159CrossRefGoogle Scholar
  19. Wood, W.G. (1984), Luminescence immunoassays — Problems and possibilities J. Clin. Chem. Clin. Biochem. 22, 905–914PubMedGoogle Scholar
  20. Wood, W.G. (1985), Luminescence immunoassays in theory and practice — The state of the art. In: Immunoassay technology, Vol 1, Ed. Pal, S.B., De Gruyter, Berlin, New York, pp. 105–150Google Scholar
  21. Wood, W.G., Fricke, H., von Klitzing, L., Strasburger, C.J., Scriba, P.C. (1982), Solid phase antigen luminescence immunoassays (SPALT) for the determination of insulin, insulin antibodies and gentamicin in serum. J. Clin. Chem. Clin. Biochem, 20, 825–831PubMedGoogle Scholar
  22. Wood, W.G., Gadow, A. (1983), Immobilisation of antigens and antibodies on macro solid phases — a comparison between adsorptive and covalent binding — Part I of a critical study of macro solid phases for use in immunoassay systems. J. Clin. Chem. Clin. Biochem. 21, 789–797PubMedGoogle Scholar
  23. Wood, W.G., Hantke, U., Gross, A.J. (1985), Initiation of luminol-based luminescence by the injection of a single reagent coupled with an enhancement of light output. J. Clin. Chem. Clin. Biochem. 23, 47–49PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • William G. Wood
    • 1
  • Harald Fricke
    • 1
  • Christian J. Strasburger
    • 1
  1. 1.Klinische Laboratorien, Klinik für Innere Medizin MedizinischeUniversität zu LübeckLübeckFederal Republic of Germany

Personalised recommendations